Protocol Protocol for generating embedding-free brain organoids enriched with oligodendrocytes

被引：8

作者：

Al-mhanawi, Bahaa ^{[1
]}

Marti, Marta Boira ^{[1
]}

Morrison, Sean D. ^{[1
]}

Gupta, Pallavi ^{[1
,2
,3
]}

Alani, Maath ^{[1
]}

Noakes, Peter G. ^{[4
,5
]}

Wolvetang, Ernst J. ^{[1
]}

Shaker, Mohammed R. ^{[1
]}

机构：

[1] Univ Queensland, Australian Inst Bioengn & Nanotechnol, St Lucia, Qld 4072, Australia

[2] Univ Queensland IIT Delhi Acad Res UQIDAR, New Delhi 110016, India

[3] Indian Inst Technol Delhi, Dept Biochem Engn & Biotechnol, New Delhi 110016, India

[4] Univ Queensland, Queensland Brain Inst, St Lucia, Qld 4072, Australia

[5] Univ Queensland, Sch Biomed Sci, St Lucia, Qld 4072, Australia

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 04期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

MODEL;

D O I：

10.1016/j.xpro.2023.102725

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In response to the scarcity of advanced in vitro models dedicated to human CNS white matter research, we present a protocol to generate neuroectodermderived embedding-free human brain organoids enriched with oligodendrocytes. We describe steps for neuroectoderm differentiation, development of neural spheroids, and their transferal to Matrigel. We then detail procedures for the development, maturation, and application of oligodendrocyte-enriched brain organoids. The presence of myelin-producing cells makes these organoids useful for studying human white matter diseases, such as leukodystrophy.

引用

页数：18

共 14 条

[1] Integration-Free Induced Pluripotent Stem Cells Model Genetic and Neural Developmental Features of Down Syndrome Etiology [J].

Briggs, James A. ;

Sun, Jane ;

Shepherd, Jill ;

Ovchinnikov, Dmitry A. ;

Chung, Tung-Liang ;

Nayler, Sam P. ;

Kao, Li-Pin ;

Morrow, Carl A. ;

Thakar, Nilay Y. ;

Soo, Set-Yen ;

Peura, Teija ;

Grimmond, Sean ;

Wolvetang, Ernst J. .

STEM CELLS, 2013, 31 (03) :467-478

[2] Engineering of human brain organoids with a functional vascular-like system [J].

Cakir, Bilal ;

Xiang, Yangfei ;

Tanaka, Yoshiaki ;

Kural, Mehmet H. ;

Parent, Maxime ;

Kang, Young-Jin ;

Chapeton, Kayley ;

Patterson, Benjamin ;

Yuan, Yifan ;

He, Chang-Shun ;

Raredon, Micha Sam B. ;

Dengelegi, Jake ;

Kim, Kun-Yong ;

Sun, Pingnan ;

Zhong, Mei ;

Lee, Sangho ;

Patra, Prabir ;

Hyder, Fahmeed ;

Niklason, Laura E. ;

Lee, Sang-Hun ;

Yoon, Young-Sup ;

Park, In-Hyun .

NATURE METHODS, 2019, 16 (11) :1169-+

[3] Single-cell transcriptome analysis reveals cell lineage specification in temporal-spatial patterns in human cortical development [J].

Fan, Xiaoying ;

Fu, Yuanyuan ;

Zhou, Xin ;

Sun, Le ;

Yang, Ming ;

Wang, Mengdi ;

Chen, Ruiguo ;

Wu, Qian ;

Yong, Jun ;

Dong, Ji ;

Wen, Lu ;

Qiao, Jie ;

Wan, Xiaoqun ;

Tang, Fuchou .

SCIENCE ADVANCES, 2020, 6 (34)

[4] iPSC-derived myelinoids to study myelin biology of humans [J].

James, Owen G. ;

Selvaraj, Bhuvaneish T. ;

Magnani, Dario ;

Burr, Karen ;

Connick, Peter ;

Barton, Samantha K. ;

Vasistha, Navneet A. ;

Hampton, David W. ;

Story, David ;

Smigiel, Robert ;

Ploski, Rafal ;

Brophy, Peter J. ;

Ffrench-Constant, Charles ;

Lyons, David A. ;

Chandran, Siddharthan .

DEVELOPMENTAL CELL, 2021, 56 (09) :1346-+

[5] Pluripotent Stem Cell-Derived Cerebral Organoids Reveal Human Oligodendrogenesis with Dorsal and Ventral Origins [J].

Kim, Hyosung ;

Xu, Ranjie ;

Padmashri, Ragunathan ;

Dunaevsky, Anna ;

Liu, Ying ;

Dreyfus, Cheryl F. ;

Jiang, Peng .

STEM CELL REPORTS, 2019, 12 (05) :890-905

[6] Production of human spinal-cord organoids recapitulating neural-tube morphogenesis [J].

Lee, Ju-Hyun ;

Shin, Hyogeun ;

Shaker, Mohammed R. ;

Kim, Hyun Jung ;

Park, Si-Hyung ;

Kim, June Hoan ;

Lee, Namwon ;

Kang, Minjin ;

Cho, Subin ;

Kwak, Tae Hwan ;

Kim, Jong Woon ;

Song, Mi-Ryoung ;

Kwon, Seung-Hae ;

Han, Dong Wook ;

Lee, Sanghyuk ;

Choi, Se-Young ;

Rhyu, Im Joo ;

Kim, Hyun ;

Geum, Dongho ;

Cho, Il-Joo ;

Sun, Woong .

NATURE BIOMEDICAL ENGINEERING, 2022, 6 (04) :435-+

[7] Induction of myelinating oligodendrocytes in human cortical spheroids [J].

Madhavan, Mayur ;

Nevin, Zachary S. ;

Shick, H. Elizabeth ;

Garrison, Eric ;

Clarkson-Paredes, Cheryl ;

Karl, Molly ;

Clayton, Benjamin L. L. ;

Factor, Daniel C. ;

Allan, Kevin C. ;

Barbar, Lilianne ;

Jain, Tanya ;

Douvaras, Panagiotis ;

Fossati, Valentina ;

Miller, Robert H. ;

Tesar, Paul J. .

NATURE METHODS, 2018, 15 (09) :700-+

[8] Differentiation and maturation of oligodendrocytes in human three-dimensional neural cultures [J].

Marton, Rebecca M. ;

Miura, Yuki ;

Sloan, Steven A. ;

Li, Qingyun ;

Revah, Omer ;

Levy, Rebecca J. ;

Huguenard, John R. ;

Pasca, Sergiu P. .

NATURE NEUROSCIENCE, 2019, 22 (03) :484-+

[9] An in vivo neuroimmune organoid model to study human microglia phenotypes [J].

Schafer, Simon T. ;

Mansour, Abed AlFatah ;

Schlachetzki, Johannes C. M. ;

Pena, Monique ;

Ghassemzadeh, Saeed ;

Mitchell, Lisa ;

Mar, Amanda ;

Quang, Daphne ;

Stumpf, Sarah ;

Ortiz, Irene Santisteban ;

Lana, Addison J. ;

Baek, Clara ;

Zaghal, Raghad ;

Glass, Christopher K. ;

Nimmerjahn, Axel ;

Gage, Fred H. .

CELL, 2023, 186 (10) :2111-+

[10] Neural Epidermal Growth Factor-Like Like Protein 2 Is Expressed in Human Oligodendroglial Cell Types [J].

Shaker, Mohammed R. ;

Kahtan, Amna ;

Prasad, Renuka ;

Lee, Ju-Hyun ;

Pietrogrande, Giovanni ;

Leeson, Hannah C. ;

Sun, Woong ;

Wolvetang, Ernst J. ;

Slonchak, Andrii .

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2022, 10

← 1 2 →