RAGE engagement by SARS-CoV-2 enables monocyte infection and underlies COVID-19 severity

被引：13

作者：

Angioni, Roberta ^{[1
,2
]}

Bonfanti, Matteo ^{[3
]}

Caporale, Nicolo ^{[3
,4
,5
]}

Sanchez-Rodriguez, Ricardo ^{[1
,2
]}

Munari, Fabio ^{[1
,2
]}

Savino, Aurora ^{[3
]}

Pasqualato, Sebastiano ^{[3
]}

Buratto, Damiano ^{[6
,7
]}

Pagani, Isabel ^{[8
]}

Bertoldi, Nicole ^{[1
,2
]}

Zanon, Carlo ^{[2
]}

Ferrari, Paolo ^{[3
]}

Ricciardelli, Eugenia ^{[3
]}

Putaggio, Cristina ^{[9
]}

Ghezzi, Silvia ^{[8
]}

Elli, Francesco

Rotta, Luca

Scardua, Alessandro

Weber, Janine

Cecatiello, Valentina

Iorio, Francesco ^{[3
]}

Zonta, Francesco ^{[7
,10
]}

Cattelan, Anna Maria ^{[9
]}

Vicenzi, Elisa ^{[8
]}

Vannini, Alessandro ^{[3
]}

Molon, Barbara ^{[1
,2
]}

Villa, Carlo Emanuele ^{[3
,5
]}

Viola, Antonella ^{[1
,2
]}

Testa, Giuseppe ^{[3
,4
,5
]}

机构：

[1] Univ Padua, Dept Biomed Sci, I-35131 Padua, Italy

[2] Fdn Ist Ric Pediat Citta Speranza, I-35127 Padua, Italy

[3] Human Technopole, Viale Rita Levi Montalcini 1, I-20157 Milan, Italy

[4] Univ Milan, Dept Oncol & Hemato Oncol, Via Santa Sofia 9, I-20122 Milan, Italy

[5] IRCCS, European Inst Oncol, Dept Expt Oncol, Via Adamello 16, I-20139 Milan, Italy

[6] Zhejiang Univ, Inst Quantitat Biol, Coll Life Sci, Hangzhou 310058, Peoples R China

[7] ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China

[8] Ist Sci San Raffaele, Viral Pathogenesis & Biosafety Unit, Via Olgettina 58, I-20132 Milan, Italy

[9] Padova Univ Hosp, Infect Dis Unit, I-35128 Padua, Italy

[10] Xian Jiaotong Liverpool Univ, Dept Biol Sci, Suzhou 215123, Peoples R China

来源：

CELL REPORTS MEDICINE | 2023年 / 4卷 / 11期

关键词：

GLYCATION END-PRODUCTS; DIFFERENTIAL EXPRESSION ANALYSIS; RECEPTOR; MILD; INFLAMMATION; DISEASE; GENE; ATHEROSCLEROSIS; ENDPRODUCTS; MECHANISMS;

D O I：

10.1016/j.xcrm.2023.101266

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has fueled the COVID-19 pandemic with its enduring medical and socioeconomic challenges because of subsequent waves and long-term consequences of great concern. Here, we chart the molecular basis of COVID-19 pathogenesis by analyzing patients' immune responses at single-cell resolution across disease course and severity. This approach confirms cell subpopulation-specific dysregulation in COVID-19 across disease course and severity and identifies a severity-associated activation of the receptor for advanced glycation endproducts (RAGE) pathway in monocytes. In vitro THP1-based experiments indicate that monocytes bind the SARSCoV-2 S1-receptor binding domain (RBD) via RAGE, pointing to RAGE-Spike interaction enabling monocyte infection. Thus, our results demonstrate that RAGE is a functional receptor of SARS-CoV-2 contributing to COVID-19 severity.

引用

页数：26

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