Extraction of phycocyanin from Spirulina using Deep Eutectic Solvent Liquid Biphasic System

被引：21

作者：

Zhuang, Dingling ^{[1
]}

Chew, Kit Wayne ^{[2
]}

Teoh, Wey Yang ^{[3
]}

Al-Maari, Mohammed Abobakr Salem ^{[3
]}

Hizaddin, Hanee Farzana ^{[3
]}

Alharthi, Sarah ^{[4
]}

Show, Pau Loke ^{[5
]}

Ling, Tau Chuan ^{[1
]}

机构：

[1] Univ Malaya, Inst Biol Sci, Fac Sci, Kuala Lumpur 50603, Malaysia

[2] Nanyang Technol Univ, Sch Chem Chem Engn & Biotechnol, 62 Nanyang Dr, Singapore 637459, Singapore

[3] Univ Malaya, Fac Engn, Ctr Separat Sci & Technol, Dept Chem Engn, Kuala Lumpur 50603, Malaysia

[4] Taif Univ, Coll Sci, Dept Chem, POB 11099, Taif 21944, Saudi Arabia

[5] Khalifa Univ, Dept Chem Engn, POB 127788, Abu Dhabi, U Arab Emirates

来源：

JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS | 2023年 / 151卷

关键词：

Phycocyanin; Green Extraction; LBS; Microalgae; Deep Eutectic Solvent; C-PHYCOCYANIN; IONIC LIQUID;

D O I：

10.1016/j.jtice.2023.105125

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Background: Phycocyanin (PC) is an intracellular protein produced in Spirulina sp. and is widely used as a natural dye in the cosmetic, food, and supplements industries. But the extraction of phycocyanin was limit by properties of proteins and technologies. Here, deep eutectic solvents were found to be an effective method of extraction, circumventing any usage of harmful organic solvents.Methods: In particular, we explored the use of DES/K2HPO4 liquid biphasic system (LBS) as the extraction medium, where the extracted PC from the cell in bottom phosphate aqueous phase is selectively transferred to the top DES phase.Findings: Among the different DES, the ChCl-Urea and ChCl-Glycerol showed the highest extraction efficiencies and yields, but the Glycerol synthesized DES has bad mass diffusion problems. Through continuous single-factor optimization, we finally achieved the best extraction efficiency and yield of 94.2% and 92.0%, respectively, using ChCl-Urea/K2HPO4. This proposed solution has the potential to be produced on a large scale and is environmentally friendly for the extraction of phycocyanin.

引用

页数：8

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