Synthetic dual hormone-responsive promoters enable engineering of plants with broad-spectrum resistance

被引：10

作者：

Li, Xianbi ^{[1
,2
]}

Niu, Guoqing ^{[1
,2
]}

Fan, Yanhua ^{[1
,2
]}

Liu, Wenying ^{[1
,2
]}

Wu, Qian ^{[1
,2
]}

Yu, Chen ^{[1
,2
]}

Wang, Jian ^{[1
,2
]}

Xiao, Yuehua ^{[1
,2
]}

Hou, Lei ^{[1
,2
]}

Jin, Dan ^{[1
,2
]}

Chen, Song ^{[1
,2
]}

Hu, Rongyu ^{[1
,2
]}

Yang, Yumei ^{[1
,2
]}

Pei, Yan ^{[1
,2
]}

机构：

[1] Chongqing Key Lab Applicat & Safety Control Genet, Chongqing 400716, Peoples R China

[2] Southwest Univ, Biotechnol Res Ctr, Chongqing 400716, Peoples R China

来源：

PLANT COMMUNICATIONS | 2023年 / 4卷 / 04期

基金：

中国国家自然科学基金;

关键词：

salicylic acid; jasmonic acid; dual hormone-responsive promoter; disease resistance; plant engineer-ing; synergistic signal; DISEASE RESISTANCE; GENE-EXPRESSION; ANTIMICROBIAL PEPTIDES; METHYL JASMONATE; SALICYLIC-ACID; AUXIN RESPONSE; PR-1A GENE; CROSS-TALK; ARABIDOPSIS; DEFENSE;

D O I：

10.1016/j.xplc.2023.100596

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In plant immunity, the mutually antagonistic hormones salicylic acid (SA) and jasmonic acid (JA) are implicated in resistance to biotrophic and necrotrophic pathogens, respectively. Promoters that can respond to both SA and JA signals are urgently needed to engineer plants with enhanced resistance to a broad spectrum of pathogens. However, few natural pathogen-inducible promoters are available for this purpose. To address this problem, we have developed a strategy to synthesize dual SA-and JA-responsive promoters by combining SA-and JA-responsive cis elements based on the interaction between their cognate trans- acting factors. The resulting promoters respond rapidly and strongly to both SA and Methyl Jasmonate (MeJA), as well as different types of phytopathogens. When such a synthetic promoter was used to control expression of an antimicrobial peptide, transgenic plants displayed enhanced resistance to a diverse range of biotrophic, necrotrophic, and hemi-biotrophic pathogens. A dual-inducible promoter responsive to the antagonistic signals auxin and cytokinin was generated in a similar manner, confirming that our strategy can be used for the design of other biotically or abiotically inducible systems.

引用

页数：14

共 52 条

[1]

Brooks DM, 2005, MOL PLANT PATHOL, V6, P629, DOI [10.1111/j.1364-3703.2005.00311.x, 10.1111/J.1364-3703.2005.00311.X]

[2] Assessment of early blight (Alternaria solani) resistance in tomato using a droplet inoculation method [J].