Exposure to low-dose arsenic caused teratogenicity and upregulation of proinflammatory cytokines in zebrafish embryos

被引:7
作者
Piyushbhai, Modi Kiran [1 ]
Binesh, Ambika [2 ]
Shanmugam, S. A. [2 ]
Venkatachalam, Kaliyamurthi [2 ]
机构
[1] Tamil Nadu Dr J Jayalalithaa Fisheries Univ, Inst Fisheries Post Grad Studies, Dept Fisheries Biotechnol, OMR Campus, Chennai 603103, Tamil Nadu, India
[2] Tamil Nadu Dr J Jayalalithaa Fisheries Univ, Inst Fisheries Post Grad Studies, Dept Basic Sci, OMR Campus, Chennai 603103, Tamil Nadu, India
关键词
Zebrafish; Embryogenesis; Inorganic arsenic; Oxidative stress; TNF-alpha; OXIDATIVE STRESS; GENE-EXPRESSION; TOXICITY TEST; IN-VIVO; REPRODUCIBILITY; MECHANISMS; RESPONSES; PROTEIN; LARVAE;
D O I
10.1007/s12011-022-03418-w
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Arsenic is currently ranked as the most toxicant on the ATSDR 2015 substance priority list and is categorised as a Group 1 human carcinogen. Biota that are subjected to inorganic arsenicals through food, water, occupational or medical exposure pose a risk to the environment and to human health. The present study was carried out to investigate the toxicity caused by inorganic arsenic. After fertilisation, zebrafish embryos were exposed to sodium arsenite at several concentrations (100 nM to 600 nM) for 24 to 96 hpf. The indicators of teratogenicity (hatchability, morphological abnormalities, mortality), behavioural modifications (touch induced escape response (TIER), startle response (SR) and turning behaviour (TB)), biochemical testing (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione S transferase (GST)) and the expressions of tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and cyclooxygenase-2 (COX-2) were investigated. The aforementioned parameters were found to be altered in embryos exposed to sodium arsenite. According to the findings of the current study, even a low dose of inorganic arsenic compound caused teratogenicity, behavioural abnormalities, altered enzyme activities and the expression of proinflammatory cytokines in zebrafish embryos.
引用
收藏
页码:3487 / 3496
页数:10
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