A next-generation sequencing approach for the detection of mixed species in canned tuna

被引：18

作者：

Klapper, Regina ^{[1
]}

Velasco, Amaya ^{[2
]}

Doring, Maik ^{[1
]}

Schroeder, Ute ^{[3
]}

Sotelo, Carmen G. ^{[2
]}

Brinks, Erik ^{[4
]}

Munoz-Colmenero, Marta ^{[2
]}

机构：

[1] Fed Res Inst Nutr & Food, Max Rubner Inst, Natl Reference Ctr Authent Food, EC Baumann Str 20, D-95326 Kulmbach, Germany

[2] Inst Invest Marinas CSIC, Eduardo Cabello 6, Vigo 36208, Spain

[3] Fed Res Inst Nutr & Food, Max Rubner Inst, Dept Safety & Qual Milk & Fish Prod, Palmaille 9, D-22767 Hamburg, Germany

[4] Fed Res Inst Nutr & Food, Max Rubner Inst, Dept Microbiol & Biotechnol, Hermann Weigmann Str 1, D-24103 Kiel, Germany

来源：

FOOD CHEMISTRY-X | 2023年 / 17卷

关键词：

Amplicon sequencing; Thunnus species identification; Seafood; Food fraud; NGS; Tuna cans; POLYMERASE-CHAIN-REACTION; THUNNUS-ALBACARES; IDENTIFICATION; PRODUCTS; QUANTIFICATION;

D O I：

10.1016/j.fochx.2023.100560

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Tuna cans are relevant seafood products for which mixtures of different tuna species are not allowed according to European regulations. In order to support the prevention of food fraud and mislabelling, a next-generation sequencing methodology based on mitochondrial cytochrome b and control region markers has been tested. Analyses of defined mixtures of DNA, fresh tissue and canned tissue revealed a qualitative and, to some extent, semiquantitative identification of tuna species. While the choice of the bioinformatic pipeline had no influence in the results (p = 0.71), quantitative differences occurred depending on the treatment of the sample, marker, species, and mixture (p < 0.01). The results revealed that matrix-specific calibrators or normalization models should also be used in NGS. The method represents an important step towards a semiquantitative method for routine control of this analytically challenging food matrix. Tests of commercial samples uncovered mixed species in some cans, being not in compliance with EU regulations.

引用

页数：12

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