Patient-Derived Breast Cancer Bone Metastasis In Vitro Model Using Bone-Mimetic Nanoclay Scaffolds

被引:5
作者
Jasuja, Haneesh [1 ]
Solaymani Mohammadi, Farid [2 ]
Kim, Jiha [2 ]
Gaba, Anu [3 ]
Katti, Dinesh R. [1 ]
Katti, Kalpana S. [1 ]
机构
[1] North Dakota State Univ, Dept Civil Construct & Environm Engn, Fargo, ND 58108 USA
[2] North Dakota State Univ, Dept Biol Sci, Fargo, ND 58108 USA
[3] Sanford Roger Maris Canc Ctr, Fargo, ND 58102 USA
关键词
PROSTATE-CANCER; CELL-CULTURE; BETA-CATENIN; OSTEOBLAST DIFFERENTIATION; EPITHELIAL TRANSITION; EXPRESSION; HYDROXYAPATITE; SURVIVAL; DISEASE; DESIGN;
D O I
10.1155/2023/5753666
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The unavailability of reliable models for studying breast cancer bone metastasis is the major challenge associated with poor prognosis in advanced-stage breast cancer patients. Breast cancer cells tend to preferentially disseminate to bone and colonize within the remodeling bone to cause bone metastasis. To improve the outcome of patients with breast cancer bone metastasis, we have previously developed a 3D in vitro breast cancer bone metastasis model using human mesenchymal stem cells (hMSCs) and primary breast cancer cell lines (MCF-7 and MDAMB231), recapitulating late-stage of breast cancer metastasis to bone. In the present study, we have tested our model using hMSCs and patient-derived breast cancer cell lines (NT013 and NT023) exhibiting different characteristics. We investigated the effect of breast cancer metastasis on bone growth using this 3D in vitro model and compared our results with previous studies. The results showed that NT013 and NT023 cells exhibiting hormone-positive and triple-negative characteristics underwent mesenchymal to epithelial transition (MET) and formed tumors in the presence of bone microenvironment, in line with our previous results with MCF-7 and MDAMB231 cell lines. In addition, the results showed upregulation of Wnt-related genes in hMSCs, cultured in the presence of excessive ET-1 cytokine released by NT013 cells, while downregulation of Wnt-related genes in the presence of excessive DKK-1, released by NT023 cells, leading to stimulation and abrogation of the osteogenic pathway, respectively, ultimately mimicking different types of bone lesions in breast cancer patients.
引用
收藏
页数:10
相关论文
共 57 条
[1]   Vascular endothelial growth factor receptors in osteoclast differentiation and function [J].
Aldridge, SE ;
Lennard, TWJ ;
Williams, JR ;
Birch, MA .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 335 (03) :793-798
[2]   Nanoclay based composite scaffolds for bone tissue engineering applications [J].
Ambre A.H. ;
Katti K.S. ;
Katti D.R. .
Journal of Nanotechnology in Engineering and Medicine, 2010, 1 (03)
[3]   In situ mineralized hydroxyapatite on amino acid modified nanoclays as novel bone biomaterials [J].
Ambre, Avinash ;
Katti, Kalpana S. ;
Katti, Dinesh R. .
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2011, 31 (05) :1017-1029
[4]   Biomineralized hydroxyapatite nanoclay composite scaffolds with polycaprolactone for stem cell-based bone tissue engineering [J].
Ambre, Avinash H. ;
Katti, Dinesh R. ;
Katti, Kalpana S. .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2015, 103 (06) :2077-2101
[5]   Nanoclays mediate stem cell differentiation and mineralized ECM formation on biopolymer scaffolds [J].
Ambre, Avinash H. ;
Katti, Dinesh R. ;
Katti, Kalpana S. .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2013, 101 (09) :2644-2660
[6]   Activated β-catenin induces osteoblast differentiation of C3H10T1/2 cells and participates in BMP2 mediated signal transduction [J].
Bain, G ;
Müller, T ;
Wang, X ;
Papkoff, J .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 301 (01) :84-91
[7]   Enhanced Metastatic Potential in a 3D Tissue Scaffold toward a Comprehensive in Vitro Model for Breast Cancer Metastasis [J].
Balachander, Gowri Manohari ;
Balaji, Sai A. ;
Rangarajan, Annapoorni ;
Chatterjee, Kaushik .
ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (50) :27810-27822
[8]  
Bray L, 2014, J TISSUE ENG REGEN M, V8, P203
[9]   Breast cancer-derived Dickkopf1 inhibits osteoblast differentiation and osteoprotegerin expression: Implication for breast cancer osteolytic bone metastases [J].
Bu, Guojun ;
Lu, Wenyan ;
Liu, Chia-Chen ;
Selander, Katri ;
Yoneda, Toshiyuki ;
Hall, Christopher ;
Keller, Evan T. ;
Li, Yonghe .
INTERNATIONAL JOURNAL OF CANCER, 2008, 123 (05) :1034-1042
[10]   Mesenchymal-to-epithelial transition facilitates bladder cancer metastasis: Role of fibroblast growth factor receptor-2 [J].
Chaffer, Christine L. ;
Brennan, Janelle P. ;
Slavin, John L. ;
Blick, Tony ;
Thompson, Erik W. ;
Williams, Elizabeth D. .
CANCER RESEARCH, 2006, 66 (23) :11271-11278