Process Optimization of Microwave-Assisted Extraction of Chlorophyll, Carotenoid and Phenolic Compounds from Chlorella vulgaris and Comparison with Conventional and Supercritical Fluid Extraction

被引:35
作者
Georgiopoulou, Ioulia [1 ]
Tzima, Soultana [1 ]
Louli, Vasiliki [1 ]
Magoulas, Kostis [1 ]
机构
[1] Natl Tech Univ Athens NTUA, Sch Chem Engn, Lab Thermodynam & Transport Phenomena, Zografou Campus, Athens 15780, Greece
来源
APPLIED SCIENCES-BASEL | 2023年 / 13卷 / 04期
关键词
microalgae; Chlorella vulgaris; microwave-assisted extraction; supercritical fluid extraction; solid-liquid extraction; bioactive molecules; antioxidant activity; ANTIOXIDANT ACTIVITY; IONIC LIQUID; BIOACTIVE COMPOUNDS; MICROALGAE; LUTEIN; FLAVONOIDS; PERFORMANCE; ULTRASOUND; RECOVERY; LIPIDS;
D O I
10.3390/app13042740
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The production of bioactive products from microalgae biomass with efficient and environmentally friendly technologies is a field of great research interest. The present work focuses on the recovery of high-added value bioactive components from Chlorella vulgaris through microwave-assisted extraction (MAE) with aq. ethanol 90% v/v. The effect of extraction temperature (40-60 degrees C), duration (5-25 min), solvent-to-biomass ratio (20-90 mL(solv)/g(biom)), and microwave power (300-800 watts) was investigated regarding the extraction yield, extract's chlorophyll, carotenoid and phenolic content, and antioxidant activity. MAE optimization at 60 degrees C, 300 watts, 14 min, and 22 mL(solv)/g(biom) led to 11.14% w/w yield, 63.36 mg/g(extr) total chlorophylls, 7.06 mg/g(extr) selected carotenoids of astaxanthin, lutein and beta-carotene, 24.88 mg/g(extr) total carotenoids, 9.34 mg(GA)/g(extr) total phenolics, and 40.49 mg(extr)/mg(DPPH) IC50 (antioxidant activity indicator). Moreover, the conventional solid-liquid extraction (SLE) with aq. ethanol 90% v/v, the supercritical fluid extraction (SFE) with CO2, as well as SFE with cosolvent addition (10% w/w ethanol), were also performed for comparison purposes. The results revealed that SLE presented the highest yield. However, the non-conventional methods of MAE and SFE led to extracts of competitive or even better quality under significantly shorter extraction duration.
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页数:25
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