Modeling transcriptional regulation of the cell cycle using a novel cybernetic-inspired approach

被引:1
|
作者
Raja, Rubesh [1 ]
Khanum, Sana [1 ]
Aboulmouna, Lina [2 ]
Maurya, Mano R. [2 ]
Gupta, Shakti [2 ]
Subramaniam, Shankar [2 ,3 ,4 ,5 ]
Ramkrishna, Doraiswami [1 ]
机构
[1] Purdue Univ, Davidson Sch Chem Engn, W Lafayette, IN 47907 USA
[2] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
[3] Univ Calif San Diego, San Diego Supercomp Ctr, Dept Comp Sci & Engn, La Jolla, CA 92093 USA
[4] Univ Calif San Diego, San Diego Supercomp Ctr, Dept Cellular & Mol Med, La Jolla, CA 92093 USA
[5] Univ Calif San Diego, Grad Program Bioinformat & Syst Biol, La Jolla, CA 92093 USA
基金
美国国家科学基金会;
关键词
CALCIUM DYNAMICS; RAW-264.7; CELLS; KINETIC-MODEL; NETWORKS; GROWTH; RECONSTRUCTION; ALGORITHM; DATABASE; TOOL;
D O I
10.1016/j.bpj.2023.12.010
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Quantitative understanding of cellular processes, such as cell cycle and differentiation, is impeded by various forms of complexity ranging from myriad molecular players and their multilevel regulatory interactions, cellular evolution with multiple intermediate stages, lack of elucidation of cause-effect relationships among the many system players, and the computational complexity associated with the profusion of variables and parameters. In this paper, we present a modeling framework based on the cybernetic concept that biological regulation is inspired by objectives embedding rational strategies for dimension reduction, process stage specification through the system dynamics, and innovative causal association of regulatory events with the ability to predict the evolution of the dynamical system. The elementary step of the modeling strategy involves stage-specific objective functions that are computationally determined from experiments, augmented with dynamical network computations involving endpoint objective functions, mutual information, change-point detection, and maximal clique centrality. We demonstrate the power of the method through application to the mammalian cell cycle, which involves thousands of biomolecules engaged in signaling, transcription, and regulation. Starting with a fine-grained transcriptional description obtained from RNA sequencing measurements, we develop an initial model, which is then dynamically modeled using the cybernetic-inspired method, based on the strategies described above. The cybernetic-inspired method is able to distill the most significant interactions from a multitude of possibilities. In addition to capturing the complexity of regulatory processes in a mechanistically causal and stage-specific manner, we identify the functional network modules, including novel cell cycle stages. Our model is able to predict future cell cycles consistent with experimental measurements. We posit that this innovative framework has the promise to extend to the dynamics of other biological processes, with a potential to provide novel mechanistic insights.
引用
收藏
页码:221 / 234
页数:14
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