Highly Promiscuous Flavonoid Di-O-glycosyltransferases from Carthamus tinctorius L.

被引:5
|
作者
Xu, Xiaoyu [1 ,2 ]
Xia, Meng [2 ]
Han, Yang [2 ]
Tan, Honghu [2 ]
Chen, Yanying [2 ]
Song, Xinqi [2 ]
Yuan, Shijun [2 ]
Zhang, Yifeng [2 ]
Su, Ping [2 ]
Huang, Luqi [1 ,2 ]
机构
[1] Jiangxi Univ Chinese Med, Res Ctr Differentiat & Dev TCM Basic Theory, Academician Workstn, Nanchang 330004, Peoples R China
[2] China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing 100700, Peoples R China
来源
MOLECULES | 2024年 / 29卷 / 03期
关键词
Carthamus tinctorius L; flavonoid glycosides; di-O-glycosyltransferases; OXIDATIVE STRESS; 3-O-GLUCOSYLTRANSFERASE; PHARMACOLOGY;
D O I
10.3390/molecules29030604
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Safflower (Carthamus tinctorius L.) has been recognized for its medicinal value, but there have been limited studies on the glycosyltransferases involved in the biosynthesis of flavonoid glycosides from safflower. In this research, we identified two highly efficient flavonoid O-glycosyltransferases, CtOGT1 and CtOGT2, from safflower performing local BLAST alignment. By constructing a prokaryotic expression vector, we conducted in vitro enzymatic reactions and discovered that these enzymes were capable of catalyzing two-step O-glycosylation using substrates such as kaempferol, quercetin, and eriodictyol. Moreover, they exhibited efficient catalytic activity towards various compounds, including flavones (apigenin, scutellarein), dihydrochalcone (phloretin), isoflavones (genistein, daidzein), flavanones (naringenin, glycyrrhizin), and flavanonols (dihydrokaempferol), leading to the formation of O-glycosides. The broad substrate specificity of these enzymes is noteworthy. This study provides valuable insights into the biosynthetic pathways of flavonoid glycosides in safflower. The discovery of CtOGT1 and CtOGT2 enhances our understanding of the enzymatic processes involved in synthesizing flavonoid glycosides in safflower, contributing to the overall comprehension of secondary metabolite biosynthesis in this plant species.
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页数:15
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