The conducting state of TRPA1 modulates channel lateral mobility

被引：0
作者：
Sampieri, Alicia [1 ]
Padilla-Flores, Teresa [1 ]
Thawani, Aditya R. [2 ]
Lam, Pui-Ying [3 ,4 ]
Fuchter, Matthew J. [2 ]
Peterson, Randall [5 ]
Vaca, Luis [1 ]
机构：
[1] Univ Nacl Autonoma Mexico, Dept Biol Celular & Desarrollo, Inst Fisiol Celular, Mexico City 04510, Mexico
[2] Imperial Coll London, Dept Chem, Mol Sci Res Hub, White City Campus, London W12 OBZ, England
[3] Med Coll Wisconsin, Dept Cell Biol Neurobiol & Anat, 8701 West Watertown Plank Rd, Milwaukee, WI 53226 USA
[4] Med Coll Wisconsin, Neurosci Res Ctr, 8701 West Watertown Plank Rd, Milwaukee, WI 53226 USA
[5] Univ Utah, Coll Pharm, 30 South 2000 East, Salt Lake City, UT 84112 USA
来源：
CELL CALCIUM | 2023年 / 116卷
基金：
英国工程与自然科学研究理事会;
关键词：
TRPA1; channel; TRPswitch; Lateral mobility; Allyl isothiocyanate; Cholesterol; PLASMA-MEMBRANE; SINGLE-CHANNEL; LIPID RAFTS; DIFFUSION; RECEPTOR; DYNAMICS;
D O I：
10.1016/j.ceca.2023.102800
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We have studied Danio rerio (Zebrafish) TRPA1 channel using a method that combines single channel electrophysiological and optical recordings to evaluate lateral mobility and channel gating simultaneously in single channels. TRPA1 channel activation by two distinct chemical ligands: allyl isothiocyanate (AITC) and TRPswitch B, results in substantial reduction of channel lateral mobility at the plasma membrane. Incubation with the cholesterol sequestering agent methyl-beta-cyclodextrin (M beta CD), prevents the reduction on lateral mobility induced by the two chemical agonists. This results strongly suggest that the open conformation of TRPA1 modulates channel lateral mobility probably by facilitating the insertion of the channel into cholesterol-enriched domains at the plasma membrane.
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页数：10
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