A novel S2-derived peptide-based ELISA for broad detection of antibody against infectious bronchitis virus

被引：4

作者：

Xie, Jing ^{[1
,2
,3
]}

Meng, Xianchen ^{[1
,2
,3
]}

Zhang, Jianjun ^{[4
]}

Xie, Quan ^{[1
,2
,3
]}

Zhang, Wei ^{[4
]}

Li, Tuofan ^{[1
,2
,3
]}

Shao, Hongxia ^{[1
,2
,3
]}

Wan, Zhimin ^{[1
,2
,3
]}

Qin, Aijian ^{[1
,2
,3
]}

Ye, Jianqiang ^{[1
,2
,3
]}

机构：

[1] Yangzhou Univ, Coll Vet Med, Key Lab Jiangsu Prevent Vet Med, Key Lab Avian Prevent Med,Minist Educ, Yangzhou 225009, Jiangsu, Peoples R China

[2] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

[3] Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou 225009, Jiangsu, Peoples R China

[4] Sinopharm Yangzhou VAC Biol Engn Co Ltd, Yangzhou 225127, Jiangsu, Peoples R China

来源：

POULTRY SCIENCE | 2023年 / 102卷 / 06期

关键词：

infectious bronchitis virus; S2; pELISA; detection of antibody; serological evaluation; LINKED-IMMUNOSORBENT-ASSAY; SPIKE ECTODOMAIN; PROTEIN; ATTACHMENT; EPITOPES; DOMAIN;

D O I：

10.1016/j.psj.2023.102661

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Avian infectious bronchitis (IB) is a highly contagious disease caused by infectious bronchitis virus (IBV). Vaccination is an effective approach for controlling IBV. Therefore, reliable immune monitoring for IB is critical for poultry. In this study, a novel peptide derived from S2 protein was used to develop an enzyme -linked immunosorbent assay (ELISA) for the detection of broadly cross-reactive antibodies against IBV. The peptide-based ELISA (pELISA) showed good specific-ity and sensitivity in detecting IBV antibodies against different serotypes. A semilogarithmic regression method for determining IBV antibody titers was also established. Antibody titers detected by pELISA and calculated with this equation were statistically similar to those evaluated by indirect fluorescence assay (IFA). Moreover, the com-parison analysis showed a 96.07% compatibility between the pELISA and IDEXX ELISA. All these data demon-strate that the pELISA generated here can be as a rapid and reliable serological surveillance tool for monitoring IBV infection or vaccination.

引用

页数：7

共 20 条

[1]

Andoh K, 2018, AVIAN DIS, V62, P210, DOI 10.1637/11796-011518-reg.1

[2]

Bailey T L, 1994, Proc Int Conf Intell Syst Mol Biol, V2, P28

[3] The S2 Subunit of Infectious Bronchitis Virus Beaudette Is a Determinant of Cellular Tropism [J].