Effects of nitrogen input on soil bacterial community structure and soil nitrogen cycling in the rhizosphere soil of Lycium barbarum L.

被引:25
作者
Li, Yuekun [1 ]
Zou, Nan [2 ]
Liang, Xiaojie [1 ]
Zhou, Xuan [1 ]
Guo, Shuhan [2 ]
Wang, Yajun [1 ]
Qin, Xiaoya [1 ]
Tian, Yehan [2 ]
Lin, Jin [2 ]
机构
[1] Ningxia Acad Agr & Forestry Sci, Wolfberry Sci Res Inst, Natl Wolfberry Engn Res Ctr, Yinchuan, Peoples R China
[2] Shandong Agr Univ, Coll Plant Protect, Shandong Prov Key Lab Biol Vegetable Dis & Insect, Tai An, Peoples R China
基金
中国国家自然科学基金;
关键词
Lycium barbarum L; bacterial community; nitrogen cycle; goji berry quality; rhizosphere soil; AMMONIA-OXIDIZING BACTERIA; DIVERSITY; ARCHAEA; FATE; UREA;
D O I
10.3389/fmicb.2022.1070817
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Lycium barbarum L., goji berry, is a precious traditional Chinese medicine and it is homology of medicine and food. Its growth is heavily dependent on nitrogen. The use of chemical fertilizers has significantly promoted the yield of goji berry and the development of the L. barbarum L. industry. However, crop plants are inefficient in the acquisition and utilization of applied nitrogen, it often leads to excessive application of nitrogen fertilizers by producers, which cause negatively impact to the environment ultimately. The exploration of an interaction model which deals with crops, chemical fertilizers, and rhizosphere microbes to improve nitrogen use efficiency, is, therefore, an important research objective to achieve sustainable development of agriculture greatly. In our study, we explored the effects of nitrogen input on soil microbial community structure, soil nitrogen cycling, and the contents of nutrients in L. barbarum fruits. The structure and composition of the bacterial community in the rhizosphere soil of L. barbarum were significantly different under different nitrogen supply conditions, and high nitrogen addition inhibited the diversity and stability of bacterial communities. Low nitrogen input stimulated the relative abundance of ammonia-oxidizing bacteria (AOB), such as Nitrosospira, catalyzing the first step of the ammonia oxidation process. The results of the GLMM model showed that the level of nitrogen fertilizer (urea) input, the relative abundance of AOB, the relative abundance of Bradyrhizobium, and their combinations had significant effects on the soil nitrogen cycling and contents of nutrients in L. barbarum fruits. Therefore, we believe that moderately reducing the use of urea and other nitrogen fertilizers is more conducive to improving soil nitrogen use efficiency and Goji berry fruit quality by increasing the nitrogen cycling potential of soil microorganisms.
引用
收藏
页数:16
相关论文
共 41 条
[1]   Fate of nitrogen in agriculture and environment: agronomic, eco-physiological and molecular approaches to improve nitrogen use efficiency [J].
Anas, Muhammad ;
Liao, Fen ;
Verma, Krishan K. ;
Sarwar, Muhammad Aqeel ;
Mahmood, Aamir ;
Chen, Zhong-Liang ;
Li, Qiang ;
Zeng, Xu-Peng ;
Liu, Yang ;
Li, Yang-Rui .
BIOLOGICAL RESEARCH, 2020, 53 (01)
[2]   Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2 [J].
Bolyen, Evan ;
Rideout, Jai Ram ;
Dillon, Matthew R. ;
Bokulich, NicholasA. ;
Abnet, Christian C. ;
Al-Ghalith, Gabriel A. ;
Alexander, Harriet ;
Alm, Eric J. ;
Arumugam, Manimozhiyan ;
Asnicar, Francesco ;
Bai, Yang ;
Bisanz, Jordan E. ;
Bittinger, Kyle ;
Brejnrod, Asker ;
Brislawn, Colin J. ;
Brown, C. Titus ;
Callahan, Benjamin J. ;
Caraballo-Rodriguez, Andres Mauricio ;
Chase, John ;
Cope, Emily K. ;
Da Silva, Ricardo ;
Diener, Christian ;
Dorrestein, Pieter C. ;
Douglas, Gavin M. ;
Durall, Daniel M. ;
Duvallet, Claire ;
Edwardson, Christian F. ;
Ernst, Madeleine ;
Estaki, Mehrbod ;
Fouquier, Jennifer ;
Gauglitz, Julia M. ;
Gibbons, Sean M. ;
Gibson, Deanna L. ;
Gonzalez, Antonio ;
Gorlick, Kestrel ;
Guo, Jiarong ;
Hillmann, Benjamin ;
Holmes, Susan ;
Holste, Hannes ;
Huttenhower, Curtis ;
Huttley, Gavin A. ;
Janssen, Stefan ;
Jarmusch, Alan K. ;
Jiang, Lingjing ;
Kaehler, Benjamin D. ;
Bin Kang, Kyo ;
Keefe, Christopher R. ;
Keim, Paul ;
Kelley, Scott T. ;
Knights, Dan .
NATURE BIOTECHNOLOGY, 2019, 37 (08) :852-857
[3]   Nitrate reduction and the nitrogen cycle in archaea [J].
Cabello, P ;
Roldán, MD ;
Moreno-Vivián, C .
MICROBIOLOGY-SGM, 2004, 150 :3527-3546
[4]  
Callahan BJ, 2016, NAT METHODS, V13, P581, DOI [10.1038/NMETH.3869, 10.1038/nmeth.3869]
[5]   The Evolution and Future of Earth's Nitrogen Cycle [J].
Canfield, Donald E. ;
Glazer, Alexander N. ;
Falkowski, Paul G. .
SCIENCE, 2010, 330 (6001) :192-196
[6]   Illumina MiSeq Sequencing Reveals Correlations among Fruit Ingredients, Environmental Factors, and AMF Communities in Three Lycium Barbarum Producing Regions of China [J].
Chen, Kaili ;
Huang, Gang ;
Li, Yuekun ;
Zhang, Xinrui ;
Lei, Yonghui ;
Li, Yang ;
Xiong, Jie ;
Sun, Yanfei .
MICROBIOLOGY SPECTRUM, 2022, 10 (02)
[7]   Root-associated microbiomes of wheat under the combined effect of plant development and nitrogen fertilization [J].
Chen, Shuaimin ;
Waghmode, Tatoba R. ;
Sun, Ruibo ;
Kuramae, Eiko E. ;
Hu, Chunsheng ;
Liu, Binbin .
MICROBIOME, 2019, 7 (01)
[8]   A Novel Integrated Method for Large-Scale Detection, Identification, and Quantification of Widely Targeted Metabolites: Application in the Study of Rice Metabolomics [J].
Chen, Wei ;
Gong, Liang ;
Guo, Zilong ;
Wang, Wensheng ;
Zhang, Hongyan ;
Liu, Xianqing ;
Yu, Sibin ;
Xiong, Lizhong ;
Luo, Jie .
MOLECULAR PLANT, 2013, 6 (06) :1769-1780
[9]   Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean [J].
Francis, CA ;
Roberts, KJ ;
Beman, JM ;
Santoro, AE ;
Oakley, BB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (41) :14683-14688
[10]   Quantitative analyses of the composition and abundance of ammonia-oxidizing archaea and ammonia-oxidizing bacteria in eight full-scale biological wastewater treatment plants [J].
Gao, Jing-Feng ;
Luo, Xin ;
Wu, Gui-Xia ;
Li, Ting ;
Peng, Yong-Zhen .
BIORESOURCE TECHNOLOGY, 2013, 138 :285-296