Long-term expansion of human hematopoietic stem cells

被引：3

作者：

Liang, Guixian ^{[1
,2
]}

Liu, Feng ^{[1
,2
,3
]}

机构：

[1] Univ Chinese Acad Sci, Chinese Acad Sci, Inst Zool, State Key Lab Membrane Biol, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing 100101, Peoples R China

[3] Shandong Univ, Sch Life Sci, Qingdao 266237, Peoples R China

来源：

CELL REGENERATION | 2023年 / 12卷 / 01期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1186/s13619-023-00163-w

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Hematopoietic stem cells (HSCs) are critical for the treatment of a variety of hematological diseases. However, the low number of HSCs lead to the clinical application difficult. To gain more functional human HSCs ex vivo, Sakurai et al. established a recombinant-cytokine-free and albumin-free culture system, i.e. PCL-PVAc-PEG-based culture, in combination with 740Y-P, butyzamide and UM171, to improve the long-term expansion of human cord blood HSCs.

引用

页数：3

共 10 条

[1] Blood Development: Hematopoietic Stem Cell Dependence and Independence [J].

Dzierzak, Elaine ;

Bigas, Anna .

CELL STEM CELL, 2018, 22 (05) :639-651

[2] Pyrimidoindole derivatives are agonists of human hematopoietic stem cell self-renewal [J].

Fares, Iman ;

Chagraoui, Jalila ;

Gareau, Yves ;

Gingras, Stephane ;

Ruel, Rejean ;

Mayotte, Nadine ;

Csaszar, Elizabeth ;

Knapp, David J. H. F. ;

Miller, Paul ;

Ngom, Mor ;

Imren, Suzan ;

Roy, Denis-Claude ;

Watts, Kori L. ;

Kiem, Hans-Peter ;

Herrington, Robert ;

Iscove, Norman N. ;

Humphries, R. Keith ;

Eaves, Connie J. ;

Cohen, Sandra ;

Marinier, Anne ;

Zandstra, Peter W. ;

Sauvageau, Guy .

SCIENCE, 2014, 345 (6203) :1509-1512

[3] Identification of HSC/MPP expansion units in fetal liver by single-cell spatiotemporal transcriptomics [J].

Gao, Suwei ;

Shi, Qiang ;

Zhang, Yifan ;

Liang, Guixian ;

Kang, Zhixin ;

Huang, Baofeng ;

Ma, Dongyuan ;

Wang, Lu ;

Jiao, Jianwei ;

Fang, Xiangdong ;

Xu, Cheng-Ran ;

Liu, Longqi ;

Xu, Xun ;

Gottgens, Berthold ;

Li, Cheng ;

Liu, Feng .

CELL RESEARCH, 2022, 32 (01) :38-53

[4] TFEB-mediated endolysosomal activity controls human hematopoietic stem cell fate [J].

Garcia-Prat, Laura ;

Kaufmann, Kerstin B. ;

Schneiter, Florin ;

Voisin, Veronique ;

Murison, Alex ;

Chen, Jocelyn ;

Chan-Seng-Yue, Michelle ;

Gan, Olga, I ;

McLeod, Jessica L. ;

Smith, Sabrina A. ;

Shoong, Michelle C. ;

Parris, Darrien ;

Pan, Kristele ;

Zeng, Andy G. X. ;

Krivdova, Gabriela ;

Gupta, Kinam ;

Takayanagi, Shin-Ichiro ;

Wagenblast, Elvin ;

Wang, Weijia ;

Lupien, Mathieu ;

Schroeder, Timm ;

Xie, Stephanie Z. ;

Dick, John E. .

CELL STEM CELL, 2021, 28 (10) :1838-+

[5] De novo generation of macrophage from placenta-derived hemogenic endothelium [J].

Liang, Guixian ;

Zhou, Chunyu ;

Jiang, Xiangxiang ;

Zhang, Yifan ;

Huang, Baofeng ;

Gao, Suwei ;

Kang, Zhixin ;

Ma, Dongyuan ;

Wang, Fengchao ;

Gottgens, Berthold ;

Wang, Hongmei ;

Han, Jing-Dong J. ;

Liu, Feng .

DEVELOPMENTAL CELL, 2021, 56 (14) :2121-+

[6] Engineering Hematopoietic Stem Cells: Lessons from Development [J].

Rowe, R. Grant ;

Mandelbaum, Joseph ;

Zon, Leonard I. ;

Daley, George Q. .

CELL STEM CELL, 2016, 18 (06) :707-720

[7] Chemically defined cytokine-free expansion of human haematopoietic stem cells [J].

Sakurai, Masatoshi ;

Ishitsuka, Kantaro ;

Ito, Ryoji ;

Wilkinson, Adam C. ;

Kimura, Takaharu ;

Mizutani, Eiji ;

Nishikii, Hidekazu ;

Sudo, Kazuhiro ;

Becker, Hans Jiro ;

Takemoto, Hiroshi ;

Sano, Tsubasa ;

Kataoka, Keisuke ;

Takahashi, Satoshi ;

Nakamura, Yukio ;

Kent, David G. ;

Iwama, Atsushi ;

Chiba, Shigeru ;

Okamoto, Shinichiro ;

Nakauchi, Hiromitsu ;

Yamazaki, Satoshi .

NATURE, 2023, 615 (7950) :127-+

[8] Haematopoietic stem cell self-renewal in vivo and ex vivo [J].

Wilkinson, Adam C. ;

Igarashi, Kyomi J. ;

Nakauchi, Hiromitsu .

NATURE REVIEWS GENETICS, 2020, 21 (09) :541-554

[9] Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation [J].

Wilkinson, Adam C. ;

Ishida, Reiko ;

Kikuchi, Misako ;

Sudo, Kazuhiro ;

Morita, Maiko ;

Crisostomo, Ralph Valentine ;

Yamamoto, Ryo ;

Loh, Kyle M. ;

Nakamura, Yukio ;

Watanabe, Motoo ;

Nakauchi, Hiromitsu ;

Yamazaki, Satoshi .

NATURE, 2019, 571 (7763) :117-+

[10] A single-cell resolution developmental atlas of hematopoietic stem and progenitor cell expansion in zebrafish [J].

Xia, Jun ;

Kang, Zhixin ;

Xue, Yuanyuan ;

Ding, Yanyan ;

Gao, Suwei ;

Zhang, Yifan ;

Lv, Peng ;

Wang, Xinyu ;

Ma, Dongyuan ;

Wang, Lu ;

Han, Jing-Dong J. ;

Liu, Feng .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2021, 118 (14)

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