Heterogeneity of fibroblasts is a hallmark of age-associated erectile dysfunction

被引:4
|
作者
Liu, Qiwei [1 ,2 ]
Song, Yulong [3 ]
Cui, Yubin [1 ]
Hu, Cheng [1 ]
Luo, Yun [1 ]
Hu, Daoyuan [1 ]
Wang, Hua [1 ]
Li, Ke [1 ]
Chen, Jun [4 ]
Xiao, Hengjun [1 ]
机构
[1] Sun Yat Sen Univ, Affiliated Hosp 3, Dept Urol, Guangzhou 510630, Peoples R China
[2] Chinese Acad Med Sci & Peking Union Med Coll, Plast Surg Inst, Beijing 100040, Peoples R China
[3] Sun Yat Sen Univ, Sch Life Sci, Key Lab Gene Engn, State Key Lab Biocontrol,Minist Educ, Guangzhou 510275, Peoples R China
[4] Sun Yat Sen Univ, Affiliated Hosp 3, Dept Infertil & Sexual Med, Guangzhou 510630, Peoples R China
来源
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2023年 / 156卷
基金
中国国家自然科学基金;
关键词
Penile corpus cavernosum; erectile dysfunction; single cell RNA sequencing; Fibroblast; CELLULAR SENESCENCE; SECRETORY PHENOTYPE; RNA-SEQ; EXPRESSION; MECHANISM; DISEASE; GROWTH; CELLS;
D O I
10.1016/j.biocel.2022.106343
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: The prevalence of age-associated erectile dysfunction (ED) increases pronouncedly with age. However, the cellular composition and transcriptomic changes of aging penile corpus cavernosum remain largely unclear. Methods: Herein, we performed single cell sequencing penile corpus cavernosum from five young with normal erectile response and five old rats with ED. Results: Clustering analysis identified 19 cell types, such as fibroblasts, myofibroblasts and immune cells. We next revealed their transcriptomic alterations and investigated novel subpopulations of major cell types. Among them, fibroblasts possessed the largest cell number and showed apparent heterogeneity. By performing single-cell entropy analysis on fibroblasts, we observed the age-associated decrease of entropy, and aged fibroblasts were found to adopt senescent secretory phenotype, as evidenced by the high expression of genes associated with the senescence-associated secretory phenotype (SASP). Finally, we constructed a comprehensive intercellular communication network and highlighted key mediators of crosstalk between fibroblasts and other cell types. Conclusions: We plotted a cellular atlas of aging cells within penile corpus cavernosum, especially fibroblasts. Our work will deepen the understanding of the heterogeneity among certain cell types within aged penile corpus cavernosum, which will generate positive effects on the future treatment of age-associated ED.
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收藏
页数:12
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