Construction of High-Density Genetic Map and QTL Mapping for Grain Shape in the Rice RIL Population

被引：1

作者：

Wei, Minyi ^{[1
]}

Luo, Tongping ^{[1
]}

Huang, Dahui ^{[1
,2
]}

Ma, Zengfeng ^{[1
]}

Liu, Chi ^{[1
]}

Qin, Yuanyuan ^{[3
]}

Wu, Zishuai ^{[1
]}

Zhou, Xiaolong ^{[1
]}

Lu, Yingping ^{[4
]}

Yan, Liuhui ^{[1
,4
]}

Qin, Gang ^{[1
]}

Zhang, Yuexiong ^{[1
,2
]}

机构：

[1] Guangxi Acad Agr Sci, Rice Res Inst, Guangxi Key Lab Rice Genet & Breeding, Nanning 530007, Peoples R China

[2] State Key Lab Conservat & Utilizat Subtrop Agrobio, Nanning 530004, Peoples R China

[3] Guangxi Acad Agr Sci, Agr Sci & Technol Informat Res Inst, Nanning 530007, Peoples R China

[4] Guangxi Acad Agr Sci, Liuzhou Res Ctr Agr Sci, Liuzhou Branch, Liuzhou 545000, Peoples R China

来源：

PLANTS-BASEL | 2023年 / 12卷 / 16期

基金：

中国国家自然科学基金;

关键词：

rice; recombinant inbred lines; high-density genetic map; grain shape; QTL mapping; RARE ALLELE; SIZE; WEIGHT; YIELD; IDENTIFICATION; ENCODES; TRAITS; GROWTH; LENGTH; BASES;

D O I：

10.3390/plants12162911

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Grain shape is an important agronomic trait directly associated with yield in rice. In order to explore new genes related to rice grain shape, a high-density genetic map containing 2193 Bin markers (526957 SNP) was constructed by whole-genome resequencing of 208 recombinant inbred (RILs) derived from a cross between ZP37 and R8605, with a total genetic distance of 1542.27 cM. The average genetic distance between markers was 0.76 cM, and the physical distance was 201.29 kb. Quantitative trait locus (QTL) mapping was performed for six agronomic traits related to rice grain length, grain width, length-to-width ratio, thousand-grain weight, grain cross-sectional area, and grain perimeter under three different environments. A total of 39 QTLs were identified, with mapping intervals ranging from 8.1 kb to 1781.6 kb and an average physical distance of 517.5 kb. Among them, 15 QTLs were repeatedly detected in multiple environments. Analysis of the genetic effects of the identified QTLs revealed 14 stable genetic loci, including three loci that overlapped with previously reported gene positions, and the remaining 11 loci were newly identified loci associated with two or more environments or traits. Locus 1, Locus 3, Locus 10, and Locus 14 were novel loci exhibiting pleiotropic effects on at least three traits and were detected in multiple environments. Locus 14, with a contribution rate greater than 10%, influenced grain width, length-to-width ratio, and grain cross-sectional area. Furthermore, pyramiding effects analysis of three stable genetic loci showed that increasing the number of QTL could effectively improve the phenotypic value of grain shape. Collectively, our findings provided a theoretical basis and genetic resources for the cloning, functional analysis, and molecular breeding of genes related to rice grain shape.

引用

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