Identification and Functional Characterization of Fungal Chalcone Synthase and Chalcone Isomerase

被引:9
|
作者
Furumura, Sho [1 ]
Ozaki, Taro [1 ]
Sugawara, Akihiro [1 ]
Morishita, Yohei [1 ]
Tsukada, Kento [1 ]
Ikuta, Tatsuya [1 ]
Inoue, Asuka [1 ]
Asai, Teigo [1 ]
机构
[1] Tohoku Univ, Grad Sch Pharmaceut Sci, Sendai 9808578, Japan
来源
JOURNAL OF NATURAL PRODUCTS | 2023年 / 86卷 / 02期
关键词
THIOESTERASE; GENES; BIOSYNTHESIS; SPECIFICITY; FLAVONOIDS; DISCOVERY; CLUSTER;
D O I
10.1021/acs.jnatprod.2c01027
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
By mining fungal genomic information, a non-canonical iterative type I PKS fused with an N-terminal adenylation-thiolation didomain, which catalyzes the formation of naringenin chalcone, was found. Structural prediction and molecular docking analysis indicated that a C-terminal thioesterase domain was involved in the Claisen-type cyclization. An enzyme responsible for formation of (2S)-flavanone in the biosynthesis of fungal flavonoids was also identified. Collectively, these findings demonstrate unprecedented fungal biosynthetic machinery leading to plant-like metabolites. fungal CHS A T I KS S o AT DH KR ACP TE R funaal QHI HO OH O OH O chalcones (2S)-flavanones
引用
收藏
页码:398 / 405
页数:8
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