Polyaniline coated plastic optic fiber biosensor for detection of aflatoxin B1 in nut, cereals, beverages, and body fluids

被引：26

作者：

Pal, Tathagata ^{[1
]}

Aditya, Simhadri ^{[1
]}

Mathai, Tennyson ^{[1
]}

Mukherji, Soumyo ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Biosci & Bioengn, Mumbai 400076, India

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2023年 / 389卷

关键词：

Aflatoxin B1; Antibody; Maximum residue limit (MRL); Plastic optic fiber biosensor; Polyaniline; U bent optical fiber; SENSOR; POLYMER;

D O I：

10.1016/j.snb.2023.133897

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Detection of aflatoxin B1 (AFB1) in environmental samples and body fluids is necessary for taking effective precautions and treatment of contamination. Its invisible presence is also associated with huge economic losses to nations. Plastic optical fiber (POF) offers a cost-effective yet flexible waveguide for visible light to develop optical biosensors. We have developed an ultrasensitive POF based AFB1 sensor that operates from pico to micromolar concentration with reasonable accuracy (R2 = 0.99). AntiAFB1 antibody has been used as bio-receptor for the sensor. The optical property of polyaniline (PAni) has been explored on polymethylmethacrylate (PMMA) surfaces of POF for the fabrication of a simple, inexpensive, yet highly sensitive AFB1 biosensor. The evanescent wave absorbance-based sensor was able to detect analytes below the maximum permissible residual limit (MRL) set by any country for spiked peanuts, corn, wheat, and beer. The sensor was further optimized to detect AFB1 in spiked human serum and urine within the pathological relevance. The recovery results fell be-tween 81 % and 117 %, demonstrating reasonable accuracy. The matrix factor of different real matrices lies within around 0.85-1.14, i.e., within the acceptable range.

引用

页数：11

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