A spatially resolved single-cell genomic atlas of the adult human breast

被引：102

作者：

Kumar, Tapsi ^{[1
,2
]}

Nee, Kevin ^{[3
]}

Wei, Runmin ^{[1
]}

He, Siyuan ^{[1
,2
]}

Nguyen, Quy H. ^{[3
]}

Bai, Shanshan ^{[1
]}

Blake, Kerrigan ^{[4
,5
]}

Pein, Maren ^{[3
,4
]}

Gong, Yanwen ^{[3
,5
]}

Sei, Emi ^{[1
]}

Hu, Min ^{[1
]}

Casasent, Anna K. ^{[1
]}

Thennavan, Aatish ^{[1
]}

Li, Jianzhuo ^{[1
]}

Tran, Tuan ^{[1
]}

Chen, Ken ^{[6
]}

Nilges, Benedikt ^{[7
]}

Kashikar, Nachiket ^{[7
]}

Braubach, Oliver ^{[8
]}

Ben Cheikh, Bassem ^{[8
]}

Nikulina, Nadya ^{[8
]}

Chen, Hui ^{[9
]}

Teshome, Mediget ^{[10
]}

Menegaz, Brian ^{[11
]}

Javaid, Huma ^{[11
]}

Nagi, Chandandeep ^{[11
]}

Montalvan, Jessica ^{[11
]}

Lev, Tatyana ^{[4
,5
]}

Mallya, Sharmila ^{[4
]}

Tifrea, Delia F. ^{[12
]}

Edwards, Robert ^{[12
]}

Lin, Erin ^{[12
]}

Parajuli, Ritesh ^{[12
]}

Hanson, Summer ^{[13
]}

Winocour, Sebastian ^{[14
]}

Thompson, Alastair ^{[14
]}

Lim, Bora ^{[15
]}

Lawson, Devon A. ^{[4
]}

Kessenbrock, Kai ^{[3
]}

Navin, Nicholas ^{[1
,2
,6
]}

机构：

[1] UT MD Anderson Canc Ctr, Dept Syst Biol, Houston, TX 77030 USA

[2] Univ Texas MD Anderson Canc Ctr, Grad Sch Biomed Sci, Houston, TX 77030 USA

[3] Univ Calif Irvine, Dept Biol Chem, Irvine, CA 92697 USA

[4] Univ Calif Irvine, Dept Physiol & Biophys, Irvine, CA 92697 USA

[5] Univ Calif Irvine, Math Computat & Syst Biol, Irvine, CA USA

[6] UT MD Anderson Canc Ctr, Dept Bioinformat & Computat Biol, Houston, TX 77030 USA

[7] Resolve Biosci, Monheim, Germany

[8] Akoya Biosci, Menlo Pk, CA USA

[9] UT MD Anderson Canc Ctr, Dept Pathol, Houston, TX USA

[10] UT MD Anderson Canc Ctr, Dept Breast Surg Oncol, Houston, TX USA

[11] Baylor Med Coll, Dept Pathol & Immunol, Houston, TX USA

[12] Univ Calif Irvine, Chao Comprehens Canc Ctr, Irvine, CA USA

[13] Univ Chicago Med, Dept Surg, Chicago, IL USA

[14] Baylor Coll Med, Dept Surg, Houston, TX USA

[15] Baylor Coll Med, Dept Med, Sect Hematol & Oncol, Houston, TX USA

来源：

NATURE | 2023年 / 620卷 / 7972期

关键词：

MAMMARY-GLAND; GENE-EXPRESSION; HIERARCHY; PERICYTES; ROLES;

D O I：

10.1038/s41586-023-06252-9

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The adult human breast is comprised of an intricate network of epithelial ducts and lobules that are embedded in connective and adipose tissue(1-3). Although most previous studies have focused on the breast epithelial system(4-6), many of the non-epithelial cell types remain understudied. Here we constructed the comprehensive Human Breast Cell Atlas (HBCA) at single-cell and spatial resolution. Our single-cell transcriptomics study profiled 714,331 cells from 126 women, and 117,346 nuclei from 20 women, identifying 12 major cell types and 58 biological cell states. These data reveal abundant perivascular, endothelial and immune cell populations, and highly diverse luminal epithelial cell states. Spatial mapping using four different technologies revealed an unexpectedly rich ecosystem of tissue-resident immune cells, as well as distinct molecular differences between ductal and lobular regions. Collectively, these data provide a reference of the adult normal breast tissue for studying mammary biology and diseases such as breast cancer.

引用

页码：181 / +

页数：37

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