Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process

被引：1
作者：
Tu, Zhifen [1 ,2 ,3 ]
Bi, Yan [3 ,4 ]
Mao, Tengyan [1 ,2 ,3 ]
Wang, Hong [1 ,2 ,3 ,4 ]
Gao, Shaorong [1 ,2 ,3 ,4 ]
Wang, Yixuan [1 ,2 ,3 ]
机构：
[1] Tongji Univ, Shanghai East Hosp, Translat Med Ctr Stem Cell Therapy, Sch Life Sci & Technol, Shanghai 200120, Peoples R China
[2] Tongji Univ, Shanghai East Hosp, Inst Regenerat Med, Sch Life Sci & Technol, Shanghai 200120, Peoples R China
[3] Tongji Univ, Frontier Sci Ctr Stem Cell Res, 1239 Siping Rd, Shanghai 200092, Peoples R China
[4] Tongji Univ, Shanghai Key Lab Maternal Fetal Med, Clin & Translat Res Ctr, Shanghai Matern & Infant Hosp 1,Sch Life Sci & Tec, Shanghai 200092, Peoples R China
来源：
CELL REGENERATION | 2023年 / 12卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Naive pluripotency; Primed-to-naive transition; Dual reporter system; Discordance between chromatin accessibility and transcriptional activity; Histone modifications; STEM-CELLS; REGULATORY ELEMENTS; NAIVE; STATE; INDUCTION; LANDSCAPE; IDENTIFICATION; GENERATION; CIRCUITRY; ALIGNMENT;
D O I：
10.1186/s13619-023-00179-2
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Naive pluripotent state can be obtained by several strategies from various types of cells, in which the cell fate roadmap as well as key biological events involved in the journey have been described in detail. Here, we carefully explored the chromatin accessibility dynamics during the primed-to-naive transition by adopting a dual fluorescent reporter system and the assay for transposase-accessible chromatin (ATAC)-seq. Our results revealed critical chromatin remodeling events and highlight the discordance between chromatin accessibility and transcriptional activity. We further demonstrate that the differential epigenetic modifications and transcription factor (TF) activities may play a critical role in regulating gene expression, and account for the observed variations in gene expression despite similar chromatin landscapes.
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页数：13
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