Determination of the Prevalence of Microsatellite Instability, BRAF and KRAS/NRAS Mutation Status in Patients with Colorectal Cancer in Slovakia

被引：0
|
作者：
Rendek, Tomas [1 ]
Saade, Rami [2 ]
Pos, Ondrej [3 ,4 ]
Kolnikova, Georgina [5 ]
Urbanova, Monika [5 ]
Budis, Jaroslav [3 ,4 ]
Mihok, Luboslav [6 ]
Tomas, Miroslav [7 ]
Szemes, Tomas [3 ,4 ]
Repiska, Vanda [1 ]
机构：
[1] Comenius Univ, Inst Med Biol Genet & Clin Genet, Fac Med, Bratislava 81108, Slovakia
[2] Comenius Univ, Fac Med, Dept Gynaecol & Obstet 2, Bratislava 81108, Slovakia
[3] Geneton Ltd, Bratislava 84104, Slovakia
[4] Comenius Univ, Sci Pk, Bratislava 84104, Slovakia
[5] NCI, Dept Pathol Anat, Bratislava 83310, Slovakia
[6] NCI, Dept Med Genet, Bratislava 83310, Slovakia
[7] Slovak Med Univ, NCI, Surg Oncol Clin, Bratislava 83310, Slovakia
来源：
CANCERS | 2024年 / 16卷 / 06期
关键词：
microsatellite instability; colorectal cancer; BRAF; KRAS; NRAS mutations; RAS MUTATIONS; KRAS; BENEFIT;
D O I：
10.3390/cancers16061128
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Slovakia has one of the highest rates of colorectal cancer among the developed countries, ranking as the second highest in the incidence of this disease for men worldwide. Despite the significant burden on both quality of life and the healthcare system this disease imposes, data on molecular analysis of biomarkers in CRC-diagnosed patients is scarce. In our study, we analyzed confirmed CRC patients from the database of the National Cancer Institute (NCI) and evaluated the presence of 4 biomarkers in tumor tissues. Altogether, 83 FFPE tumor tissues from CRC patients listed in the NCI database were analyzed for microsatellite instability status, presence of BRAF and KRAS/NRAS mutations, and neoplastic cell percentage in tissue samples. We identified 4 MSI-high samples, 39 KRAS/NRAS mutations, and 5 BRAF p.V600E mutations, with one case of coexistence of all three markers in a single tumor sample. We also evaluated possible relationships between biomarkers, their coexistence, and the age and sex of the studied population.
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页数：10
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