ISSR and SRAP for assessing genetic variability of Indonesian local rice genotypes (Oryza sativa L.)

被引：2

作者：

Rini, Dwi Setyo ^{[1
]}

Budiyanti, Yashinta ^{[2
]}

Valentine, Meyta ^{[2
]}

Permana, Rega ^{[3
]}

机构：

[1] Natl Res & Innovat Agcy BRIN, Res Ctr Biosystemat & Evolut, Jl Raya Jakarta Bogor Km 46, Cibinong, Indonesia

[2] Surya Univ, Dept Life Sci, F8 & F9 Grand Serpong Mall,Jalan MH Thamrin Panung, Banten 15143, Indonesia

[3] Padjadjaran State Univ, Fac Fisheries & Marine Sci, Jl Raya Bandung Sume dang KM21 Jatinangor, Sumedang 45363, Indonesia

来源：

CROP BREEDING AND APPLIED BIOTECHNOLOGY | 2023年 / 23卷 / 04期

关键词：

ISSR; genetic variability; Oryza sativa L; SRAP; RAPD;

D O I：

10.1590/1984-70332023v23n4a46

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

This study evaluated the genetic variability of 34 local rice genotypes from Simeulue Island and Sumba Island, Indonesia, using ISSR and SRAP mark-ers. The amplification of ISSR and SRAP primers produced high percentages of polymorphism, with values of 98.17% and 99.75%, respectively. The result of the UPGMA dendrogram analysis classified the rice genotypes into three groups by ISSR and two groups by SRAP. The ISSR had a higher Resolving Power (RP = 14.66) and Marker Index (MI = 4.61) than the SRAP (RP = 9.13, MI = 3.8). How-ever, the results of the UPGMA dendrogram corresponded to the STRUCTURAL data analysis. The Delta K of STRUCTURAL analysis performed its maximum value when K = 3 by ISSR and K = 2 by SRAP. This study pointed out that ISSR markers were more effective than SRAP markers in evaluating the degree of genetic variability of rice genotypes.

引用

页数：9

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