A New Method for Express Detection of Antibiotic Resistance

被引：4

作者：

Pleskova, S. N. ^{[1
,2
]}

Lazarenko, E. V. ^{[1
,2
]}

Sudakova, I. S. ^{[2
]}

Kriukov, R. N. ^{[1
]}

Bezrukov, N. A. ^{[1
,2
]}

机构：

[1] Nizhny Novgorod Lobachevsky State Univ, Minist Sci & Higher Educ, Nizhnii Novgorod 603950, Russia

[2] Nizhny Novgorod Alekseeva State Tech Univ, Minist Sci & Higher Educ, Nizhnii Novgorod 603115, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2023年 / 59卷 / 01期

基金：

俄罗斯科学基金会;

关键词：

antibiotic resistance; atomic force microscopy; oscillation mode; disk diffusion method; Escherichia coli; Staphylococcus aureus;

D O I：

10.1134/S0003683823010076

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The oscillation mode of an atomic force microscope (AFM) was used to create a highly sensitive real-time detection system for antibiotic resistance. This mode allows one to evaluate the sensitivity or resistance of Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria to an antibiotic in 15-30 minutes. The analytical signal (changes in the amplitude-frequency characteristics of the cantilever) is based on the metabolic activity of bacteria. Bacteria were placed on the cantilever and causing it to oscillate with high amplitude. If the bacteria are sensitive to the antibiotic, the amplitude drops statistically significant within 15-30 minutes, if the bacteria are resistant, then the amplitude either does not change or increases. The results were comparable with the disk diffusion method.

引用

页码：73 / 78

页数：6

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