Characterization of a Novel Superoxide Dismutase from a Deep-sea Sea Cucumber (Psychoropotes verruciaudatus)

被引：2
作者：
Li, Yanan [1 ]
Chen, Zongfu [1 ]
Zhang, Peng [2 ]
Gao, Feng [1 ]
Wang, Junfeng [3 ]
Lin, Li [1 ]
Zhang, Haibin [4 ]
机构：
[1] Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Breed, Guangzhou 510222, Peoples R China
[2] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangdong Prov Key Lab Fishery Ecol & Environm, Guangzhou 510300, Peoples R China
[3] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Peoples R China
[4] Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
来源：
ANTIOXIDANTS | 2023年 / 12卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Holothuroidea; deep sea; enzyme expression; purification; MANGANESE-SUPEROXIDE; OXIDATIVE STRESS; REACTIVE OXYGEN; CLONING; EXPRESSION; CDNA;
D O I：
10.3390/antiox12061227
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
At present, deep-sea enzymes are a research hotspot. In this study, a novel copper-zinc superoxide dismutase (CuZnSOD) was successfully cloned and characterized from a new species of sea cucumber Psychropotes verruciaudatus (PVCuZnSOD). The relative molecular weight of the PVCuZnSOD monomer is 15 kDa. The optimum temperature of PVCuZnSOD is 20 & DEG;C, and it maintains high activity in the range of 0-60 degrees C. It also has high thermal stability when incubated at 37 & DEG;C. PVCuZnSOD has a maximum activity of more than 50% in the pH range of 4-11 and a high activity at pH 11. In addition, PVCuZnSOD has strong tolerance to Ni2+, Mg2+, Ba2+, and Ca2+, and it can withstand chemical reagents, such as Tween20, TritonX-100, ethanol, glycerol, isopropanol, DMSO, urea, and GuHCl. PVCuZnSOD also shows great stability to gastrointestinal fluid compared with bovine SOD. These characteristics show that PVCuZnSOD has great application potential in medicine, food, and other products.
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页数：15
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共 41 条
  • [1] Cafeteria diet induces obesity and insulin resistance associated with oxidative stress but not with inflammation: improvement by dietary supplementation with a melon superoxide dismutase
    Carillon, Julie
    Romain, Cindy
    Bardy, Guillaume
    Fouret, Gilles
    Feillet-Coudray, Christine
    Gaillet, Sylvie
    Lacan, Dominique
    Cristol, Jean-Paul
    Rouanet, Jean-Max
    [J]. FREE RADICAL BIOLOGY AND MEDICINE, 2013, 65 : 254 - 261
  • [2] Superoxide Dismutase Enzymosomes: Carrier Capacity Optimization, in Vivo Behaviour and Therapeutic Activity
    Corvo, M. Luisa
    Marinho, H. Susana
    Marcelino, Paulo
    Lopes, Rui M.
    Vale, Carlos A.
    Marques, Claudia R.
    Martins, Luisa C. D.
    Laverman, Peter
    Storm, Gert
    Martins, M. Barbara A. F.
    [J]. PHARMACEUTICAL RESEARCH, 2015, 32 (01) : 91 - 102
  • [3] Deng D., 2016, XIANDAI SHIPIN KEJI, V32, P77
  • [4] A single center, pilot, double-blinded, randomized, comparative, prospective clinical study to evaluate improvements in the structure and function of facial skin with tazarotene 0.1% cream alone and in combination with GliSODin (R) Skin Nutrients Advanced Anti-Aging Formula
    Goldberg, Lawrence D.
    Crysler, Corina
    [J]. CLINICAL COSMETIC AND INVESTIGATIONAL DERMATOLOGY, 2014, 7 : 139 - 144
  • [5] Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue
    Hunter, T
    Bannister, JV
    Hunter, GJ
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 2002, 269 (21): : 5137 - 5148
  • [6] Cloning and characterization of iron-superoxide dismutase in Antarctic yeast strain Rhodotorula mucilaginosa AN5
    Kan, Guangfeng
    Wen, Hua
    Wang, Xiaofei
    Zhou, Ting
    Shi, Cuijuan
    [J]. JOURNAL OF BASIC MICROBIOLOGY, 2017, 57 (08) : 680 - 690
  • [7] Karuppanapandian T, 2011, AUST J CROP SCI, V5, P709
  • [8] Copper/zinc-superoxide dismutase from Epinephelus malabaricus cDNA and enzyme property
    Ken, CF
    Cheng, YF
    Chang, CF
    Lin, CT
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (19) : 5688 - 5694
  • [9] Superoxide Dismutase Multigene Family from a Primitive Chondrostean Sturgeon, Acipenser baerii: Molecular Characterization, Evolution, and Antioxidant Defense during Development and Pathogen Infection
    Kim, Chan-Hee
    Kim, Eun Jeong
    Nam, Yoon Kwon
    [J]. ANTIOXIDANTS, 2021, 10 (02) : 1 - 29
  • [10] High-level expression of a sika deer (Cervus nippon) Cu/Zn superoxide dismutase in Pichia pastoris and its characterization
    Li, Ren-Kuan
    Fu, Cai-Li
    Chen, Ping
    Ng, Tzi Bun
    Ye, Xiu-Yun
    [J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2013, 35 (02) : 185 - 192
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