Effect of mirasol pathogen reduction technology system on immunomodulatory molecules of apheresis platelets

Pa iota hogen inactivation for platelets ry wbcflavin system (MIRASOL) efflciently rea::ce,. t center dot anafusion related pattioaen transmission. However little is knonn about its impact on platelets' immunomodulatory biochemical profile. Vve aimed ,cas to assess the euects of MIRASOL treatment on platelet guality parameters and inununomodulatmy molecules CD62P, RANTES, and CD4011 in Single Donor :`latelets (SDPs) resttspended in plasina (SDP-P) or T-PAS and additive solution (SDP-A). Twenty nine SDPs (15 SDP-P and 14 SDP-A) ,vere included in the study. Samples were collected before, after MIRASOL treatment and just before transfusion. P-selectin (CD62P), RANTES, and CD40L were tested by A:LISA. Platelet prodttcts guality assays were also perfonned. Platelet count/unit decreased after Mirasol treatment by 13 %. The pH of all units decreased over the 5-day storage period but remained above expected limits and the swirlinb test was positive throughout storage. Pselectin levels were not different between the three different time points in both SDPs-P and SDPs-A while RANTES levels were found to differ statistically significantly at the three different time points in all units and in the SPD-A subgroup. CD40L levels in all SDP products increased slightly during storage but this was not statistically significant. CDb2P, RANTES, and CD40L in all time points were elevated in SDPs-A compared to SDPs-P but not at a statistically significant level. [n condusion MIRASOL treatment apart from RANTES increase does not seem to substantially affect platelets associated other cytokines and immtmomodulatmy molecules namely Pselectin and sCD40L which are implicated in immune transfusion reactions.