IDENTIFICATION OF GINSENG (PANAX GINSENG C. A. MAYER) CONTINUOUS CROPPING OBSTACLE RESPONSIVE MIRNAS AND THEIR TARGET GENES

The problem of continuous cropping obstacle has seriously restricted the development of the ginseng industry. In view of the little research on the mechanism of ginseng crop disorder, this study was conducted to investigate the ginseng crop disorder-related miRNAs and their target genes for the sustainability of the ginseng industry. A total of 376 miRNAs were obtained from all the tissue samples of the Continuous Cropping obstacles (CCO) and Control Check (CK). 121 miRNAs were found to be differentially expressed in the ginseng. Then, the target genes of differentially expressed miRNAs were enriched and found to be involved in phytohormone-related regulation, MAPK signaling pathway - plant, amino and nucleotide sugar metabolism, and monoterpene biosynthesis, suggesting that the alteration of these related pathways may play a key role in causing ginseng crop disorder. Finally, the correlations between miRNAs and mRNAs were integrated to construct the corresponding reciprocal networks and discover the miRNAs that might play a key role in causing ginseng crop disorder. osa-MIR818-dp3_1ss1TA and pgi-MIR6135i-p3_1ss11TG and pgi-MIR6140c-p5_1ss12CT were found to be at the core of the network, and it was hypothesized that these miRNAs play an important role in the formation of ginseng continuous cropping obstacle.