Effects of lactic acid bacteria fermented feed and three types of lactic acid bacteria (L. plantarum, L. acidophilus, B. animalis) on intestinal microbiota and T cell polarization (Th1, Th2, Th17, Treg) in the intestinal lymph nodes and spleens of rats

被引：6

作者：

Yu, Da Yoon ^{[1
]}

Oh, Sang-Hyon ^{[1
]}

Kim, In Sung ^{[1
]}

Ii Kim, Gwang ^{[1
]}

Kim, Jeong A. ^{[1
]}

Moon, Yang Soo ^{[2
]}

Jang, Jae Cheol ^{[1
]}

Lee, Sang Suk ^{[3
]}

Jung, Jong Hyun ^{[4
]}

Park, Hwa Chun ^{[5
]}

Cho, Kwang Keun ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, Div Anim Sci, Jinju 52725, South Korea

[2] Gyeongsang Natl Univ, Div Anim Biosci & Integrated Biotechnol, Jinju 52725, South Korea

[3] Dept Anim Sci, Sunchon 57922, South Korea

[4] Jung P&C Inst, Yongin 16950, South Korea

[5] Dasan Genet, Namwon 55716, South Korea

来源：

ANIMAL BIOSCIENCE | 2023年 / 36卷 / 01期

关键词：

Body Weight; Intestinal Microbiota; Lactic Acid Bacteria; Rubus coreanus; T Cell Polarization; GUT MICROBIOTA; MECHANISMS; OBESITY;

D O I：

10.5713/ab.22.0301

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Objective: In this study, we investigated the effects of Rubus coreanus-derived lactic acid bacteria (LAB) fermented feed (RC-LAB fermented feed) and three types of LAB (Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium animalis) on the expression of transcription factors and cytokines in Th1, Th2, Th17, and Treg cells in the intestinal lymph nodes and spleens of rats. In addition, the effect on intestinal microbiota composition and body weight was investigated. Methods: Five-week-old male rats were assigned to five treatments and eight replicates. The expression of transcription factors and cytokines of Th1, Th2, Th17, and Treg cells in the intestinal lymph nodes and spleens was analyzed using real-time reverse transcriptase polymerase chain reaction assays. Intestinal tract microbiota compositions were analyzed by next-generation sequencing and quantitative polymerase chain reaction assays. Results: RC-LAB fermented feed and three types of LAB increased the expression of transcription factors and cytokines in Th1, Treg cells and Galectin-9, but decreased in Th2 and Th17 cells. In addition, the intestinal microbiota composition changed, the body weight and Firmicutes to Bacteroidetes (F/B) ratio decreased, and the relative abundance of LAB increased. Conclusion: LAB fermented feed and three types of LAB showed an immune modulation effect by inducing T cell polarization and increased LAB in the intestinal microbiota.

引用

页码：156 / 166

页数：11