Lipid droplets imaging with three-photon microscopy

被引：8
|
作者：
He, Mubin [1 ]
Park, Hojeong [2 ]
Niu, Guangle [3 ]
Xia, Qiming [4 ]
Zhang, Hequn [1 ]
Tang, Ben Zhong [5 ]
Qian, Jun [1 ]
机构：
[1] Zhejiang Univ, State Key Lab Modern Opt Instrumentat, Ctr Opt & Electromagnet Res, Int Res Ctr Adv Photon,Coll Opt Sci & Engn, Hangzhou 310058, Peoples R China
[2] Hong Kong Univ Sci & Technol, Chinese Natl Engn Res Ctr Tissue Restorat & Recon, Dept Chem, Hong Kong Branch,Kowloon, Clear Water Bay, Hong Kong 999077, Peoples R China
[3] Shandong Univ, Shenzhen Res Inst, Shenzhen 518057, Peoples R China
[4] Zhejiang Univ, Sir Run Run Shaw Hosp, Sch Med, Dept Gen Surg, Hangzhou 310000, Peoples R China
[5] Chinese Univ Hong Kong, Sch Sci & Engn, Shenzhen Inst Aggregate Sci & Technol, Shenzhen 518172, Guangdong, Peoples R China
来源：
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES | 2023年 / 16卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Lipid droplets; three-photon fluorescence microscopy; fat liver; deep-tissue imaging; FATTY LIVER-DISEASE; LIVING CELLS; PROBE;
D O I：
10.1142/S179354582250033X
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Lipid droplets (LDs) participate in many physiological processes, the abnormality of which will cause chronic diseases and pathologies such as diabetes and obesity. It is crucial to monitor the distribution of LDs at high spatial resolution and large depth. Herein, we carried three-photon imaging of LDs in fat liver. Owing to the large three-photon absorption cross-section of the luminogen named NAP-CF3 (1:67 x 10(-79) cm(6) s(2)), three-photon fluorescence fat liver imaging reached the largest depth of 80 mu m. Fat liver diagnosis was successfully carried out with excellent performance, providing great potential for LDs-associated pathologies research.
引用
收藏
页数：9
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