Measuring the transcriptome-wide effects of aging on murine adipocytes using RNAseq

被引：0
|
作者：
Cauwer, Aurore De [1 ]
Pichot, Angelique [1 ]
Molitor, Anne [1 ]
Stemmelen, Tristan [1 ,2 ]
Carapito, Raphael [1 ,2 ]
Bahram, Seiamak [1 ,2 ]
Georgel, Philippe [1 ]
机构：
[1] Univ Strasbourg, Lab ImmunoRhumatol Mol, INSERM UMR S 1109, Inst Themat Interdisciplinaire ITI Med Precis Stra, F-67085 Strasbourg, France
[2] Nouvel Hop Civil, Lab Immunol, Plateau Tech Biol, Pole Biol, F-67000 Strasbourg, France
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 03期
关键词：
Differential analysis; Mandatory quality; final analysis;
D O I：
10.1016/j.xpro.2023.102397
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Adipose tissue plays a central role in age-related diseases. While RNAseq proto-cols exist for many tissues, few data have been generated with this technology to explore gene expression in adipocytes, particularly during aging. Here, we pre-sent a protocol to analyze the transcriptional changes that occur in adipose tissue during normal and accelerated aging in mouse models. We describe steps for genotyping, diet control, euthanasia, and dissection. We then detail RNA purifi-cation and genome-wide data generation and analysis. For complete details on the use and execution of this protocol, please refer to De Cauwer et al. (2022) iScience. Sep 16;25(10):105149.
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页数：17
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