Effects of the Ethanol and Ethyl Acetate Extracts of Terminalia chebula Retz. on Proliferation, Migration, and HIF-1a and CXCR-4 Expression in MCF-7 Cells: an In Vitro Study

被引:5
作者
Mehrabani, Mitra [1 ,2 ]
Jafarinejad-Farsangi, Saeideh [3 ]
Raeiszadeh, Mahboobeh [2 ,4 ]
Tarzi, Mojdeh Esmaeili [2 ]
Sheikholeslami, Mozhgan [5 ]
Nematollahi, Mohammad Hadi [6 ]
Khoshfekr, Vajihe [2 ]
Juybari, Kobra Bahrampour [7 ,8 ]
Mehrabani, Mehrnaz [3 ]
机构
[1] Kerman Univ Med Sci, Student Res Comm, Kerman, Iran
[2] Kerman Univ Med Sci, Herbal & Tradit Med Res Ctr, Kerman, Iran
[3] Kerman Univ Med Sci, Inst Neuropharmacol, Physiol Res Ctr, Kerman, Iran
[4] Kerman Univ Med Sci, Fac Persian Med, Dept Tradit Pharm, Kerman, Iran
[5] Univ Sistan & Baluchestan, Dept Biol, Zahedan, Iran
[6] Kerman Univ Med Sci, Inst Neuropharmacol, Neurosci Res Ctr, Kerman, Iran
[7] Semnan Univ Med Sci, Abnormal Uterine Bleeding Res Ctr, Semnan, Iran
[8] Semnan Univ Med Sci, Sch Med, Dept Pharmacol, Semnan, Iran
关键词
Cytotoxicity; Terminalia chebula Retz; MCF-7 cell line; Spheroids; HIF-1; alpha; CXCR-4; GALLIC ACID; ANTIOXIDANT ACTIVITY; SIGNALING PATHWAY; OXIDATIVE STRESS; APOPTOSIS; INHIBITION; GROWTH; GENE;
D O I
10.1007/s12010-022-04301-z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Over recent years, much attention has been devoted to the field of screening natural products and/or their novel structures because of reversing cancer progression. The current research work was intended to explore the cytotoxic activity of ethanol and ethyl acetate extracts of dried fruit of Terminalia chebula Retz. (T. chebula) in MCF-7 cell line. High-performance thin-layer chromatographic (HPTLC) method and Folin-Ciocalteu colorimetric techniques were performed. Anti-proliferative activities of T. chebula fruit extracts on the MCF-7 cell line were evaluated using MTT assay. Effects of both extracts on the migration of MCF-7 cells and the size of MCF-7-derived spheroids were also evaluated. Moreover, antioxidant properties were measured by DPPH and FRAP methods. Western blotting was used to measure the HIF-1 alpha and CXCR-4 protein levels. Chebulagic acid, gallic acid, chebulinic acid, and ellagic acid were found as major compounds in both extracts. The total phenolic contents based on gallic acid equivalent (GAE) in the ethanol and ethyl acetate extracts of T. chebula were found to be 453.68 +/- 0.31 and 495.12 +/- 0.43 mg GAE/g dry weight of the extract, respectively. Both extracts exerted a significant dose- and time-dependent cytotoxicity effect on MCF-7 cells. They also had a marked negative effect on the average size of MCF-7-derived spheroids and their migration rate. None of the extracts exhibited stronger antioxidant activities than vitamin C. Furthermore, both extracts at a concentration of 125 mu g/ml could meaningfully decrease the expression levels of HIF-1 alpha and CXCR-4 in MCF-7 cells. These data represent that T. chebula may be a valuable medicinal resource in the regulation of breast cancer proliferation, growth, and metastasis.
引用
收藏
页码:3327 / 3344
页数:18
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