Evaluating mitochondrial length, volume, and cristae ultrastructure in rare mouse adult stem cell populations

被引：4

作者：

Triolo, Matthew ^{[1
]}

Wade, Steven ^{[1
]}

Baker, Nicole ^{[1
]}

Khacho, Mireille ^{[1
]}

机构：

[1] Univ Ottawa, Ottawa Inst Syst Biol OISB, Fac Med, Ctr Neuromuscular Dis CNMD,Dept Biochem Microbiol, Ottawa, ON K1H 8M5, Canada

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

QUIESCENT;

D O I：

10.1016/j.xpro.2023.102107

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Since changes in mitochondrial morphology regulate key functions of stem cells, it is important to assess their structure under physiological and pathophysiological conditions. Here, we present techniques optimized in rare adult muscle stem cells (MuSCs). For evaluating mitochondrial length and volume within a compact cytoplasmic area in MuSCs on intact myofibers, we describe steps for mitochondrial staining, imaging, and quantification. For evaluating mitochondrial ultra structure in small cell numbers, we describe steps for agarose embedding and quantification by TEM. For complete details on generation and use of this protocol, please refer to Baker et al. (2022).1

引用

页数：20