Genomic epidemiology and transmission characteristics of mcr1-positive colistin-resistant Escherichia coli strains circulating at natural environment

被引:8
作者
Hu, Xinjun [1 ]
Chen, Yingying [2 ]
Xu, Hao [3 ]
Qiao, Jie [3 ]
Ge, Haoyu [3 ]
Liu, Ruishan [3 ]
Zheng, Beiwen [3 ]
机构
[1] Henan Univ Sci & Technol, Affiliated Hosp 1, Coll Clin Med, Dept Infect Dis, Luoyang 471000, Peoples R China
[2] Zhejiang Univ, Shaoxing Peoples Hosp, Shaoxing Hosp, Sch Med,Dept Neurosurg, Shaoxing 312000, Peoples R China
[3] Zhejiang Univ, Affiliated Hosp 1, Collaborat Innovat Ctr Diag & Treatment Infect Dis, Sch Med,State Key Lab Diag & Treatment Infect Dis, Hangzhou 310000, Peoples R China
关键词
Escherichia coli; Colistin-resistant; MCR-1; Environment; Transmission; ANTIMICROBIAL RESISTANCE; PIG FARMS; GENE MCR-1; PLASMID; STABILITY; EMERGENCE; LIVESTOCK; ANIMALS; HEALTH; CHINA;
D O I
10.1016/j.scitotenv.2023.163600
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
MCR-positive Escherichia coli (MCRPEC) have been reported in humans worldwide. The high prevalence of mcr-1 poses clinical and environmental risks due to its diverse genetic mechanisms. Given the vital role of animals and the environ-ment in the spread of antibiotic resistance, a "One Health" perspective should be taken when addressing antimicrobial resistance issues. This study conducted a prospective study in six farms (located in Jiaxing City, Zhejiang province, China) in 2019. MCRPEC strains were screened from samples of different sources. The molecular epidemiological sur-veys and transmission potential were investigated by whole-genome sequencing and phylogenetic analysis. MCRPEC were detected in different farms with various sources. Sequence type complex 10 was dominant and distributed widely in multiple sources. Core-genome multilocus sequence type (cgMLST) analysis indicated that clonal transmission could occur within and between farms. In addition, mcr-1 genes with different locations showed different transmission ten-dencies. The study indicated that interspecies and cross-regional transmission of MCRPEC could occur between differ-ent sectors in farms. Further surveillance and research of non-clinical MCRPEC strains are necessary to reduce the threat of MCRPEC.
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页数:11
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共 61 条
[1]   Burden of endemic health-care-associated infection in developing countries: systematic review and meta-analysis [J].
Allegranzi, Benedetta ;
Nejad, Sepideh Bagheri ;
Combescure, Christophe ;
Graafmans, Wilco ;
Attar, Homo ;
Donaldson, Liam ;
Pittet, Didier .
LANCET, 2011, 377 (9761) :228-241
[2]   Call of the wild: antibiotic resistance genes in natural environments [J].
Allen, Heather K. ;
Donato, Justin ;
Wang, Helena Huimi ;
Cloud-Hansen, Karen A. ;
Davies, Julian ;
Handelsman, Jo .
NATURE REVIEWS MICROBIOLOGY, 2010, 8 (04) :251-259
[3]   SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing [J].
Bankevich, Anton ;
Nurk, Sergey ;
Antipov, Dmitry ;
Gurevich, Alexey A. ;
Dvorkin, Mikhail ;
Kulikov, Alexander S. ;
Lesin, Valery M. ;
Nikolenko, Sergey I. ;
Son Pham ;
Prjibelski, Andrey D. ;
Pyshkin, Alexey V. ;
Sirotkin, Alexander V. ;
Vyahhi, Nikolay ;
Tesler, Glenn ;
Alekseyev, Max A. ;
Pevzner, Pavel A. .
JOURNAL OF COMPUTATIONAL BIOLOGY, 2012, 19 (05) :455-477
[4]   Trimmomatic: a flexible trimmer for Illumina sequence data [J].
Bolger, Anthony M. ;
Lohse, Marc ;
Usadel, Bjoern .
BIOINFORMATICS, 2014, 30 (15) :2114-2120
[5]   Escherichia coli used as a biomarker of antimicrobial resistance in pig farms of Southern Brazil [J].
Brisola, Maiara Cristiane ;
Crecencio, Regiane Boaretto ;
Bitner, Dinael Simao ;
Frigo, Angelica ;
Rampazzo, Luana ;
Stefani, Lenita Moura ;
Faria, Glaucia Amorim .
SCIENCE OF THE TOTAL ENVIRONMENT, 2019, 647 :362-368
[6]   Serotypes, genotypes and antimicrobial resistance patterns of human diarrhoeagenic Escherichia coli isolates circulating in southeastern China [J].
Chen, Y. ;
Chen, X. ;
Zheng, S. ;
Yu, F. ;
Kong, H. ;
Yang, Q. ;
Cui, D. ;
Chen, N. ;
Lou, B. ;
Li, X. ;
Tian, L. ;
Yang, X. ;
Xie, G. ;
Dong, Y. ;
Qin, Z. ;
Han, D. ;
Wang, Y. ;
Zhang, W. ;
Tang, Y-W ;
Li, L. .
CLINICAL MICROBIOLOGY AND INFECTION, 2014, 20 (01) :52-58
[7]   Genomic epidemiology of emerging ESBL-producingSalmonellaKentuckyblaCTX-M-14bin Europe [J].
Coipan, Claudia E. ;
Westrell, Therese ;
van Hoek, Angela H. A. M. ;
Alm, Erik ;
Kotila, Saara ;
Berbers, Bas ;
De Keersmaecker, Sigrid C. J. ;
Ceyssens, Pieter-Jan ;
Borg, Maria Louise ;
Chattaway, Marie ;
McCormick, Jacquelyn ;
Dallman, Timothy J. ;
Franz, Eelco .
EMERGING MICROBES & INFECTIONS, 2020, 9 (01) :2124-2135
[8]   Stability of a promiscuous plasmid in different hosts: no guarantee for a long-term relationship [J].
De Gelder, Leen ;
Ponciano, Jose M. ;
Joyce, Paul ;
Top, Eva M. .
MICROBIOLOGY-SGM, 2007, 153 :452-463
[9]   'To be, or not to be'-The dilemma of 'silent' antimicrobial resistance genes in bacteria [J].
Deekshit, Vijaya Kumar ;
Srikumar, Shabarinath .
JOURNAL OF APPLIED MICROBIOLOGY, 2022, 133 (05) :2902-2914
[10]   Multiple sequence types, virulence determinants and antimicrobial resistance genes in multidrug- and colistin-resistant Escherichia coli from agricultural and non-agricultural soils [J].
Furlan, Pedro Rueda ;
Stehling, Eliana Guedes .
ENVIRONMENTAL POLLUTION, 2021, 288