Molecular detection of Coxiella burnetii in Kope cheese and cattle milk in West Azerbaijan, Iran

被引:3
作者
Mokarizadeh, Kosar [1 ]
Ownagh, Addolghaffar [2 ]
Tajik, Hossein [3 ]
机构
[1] Urmia Univ, Fac Vet Med, Dept Microbiol, Orumiyeh, Iran
[2] Urmia Univ, Fac Vet Med, Dept Microbiol, Orumiyeh, Iran
[3] Urmia Univ, Fac Vet Med, Dept Food Hyg & Qual Control, Orumiyeh, Iran
关键词
Coxiella burnetii; Dairy; Iran; IS1111; gene; Q-FEVER; RAW-MILK; ABORTION; PCR; HISTORY; SAMPLES; SHEEP; DNA;
D O I
10.30466/vrf.2022.544750.3323
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
There are few studies on Coxiella burnetii (Cb) as a causative agent of Q fever in dairy products in Iran. The prevalence of Cb was studied by polymerase chain reaction (PCR) method in Kope (pot) cheese and cattle milk collected from West Azerbaijan province, Iran. A total number of 240 Kope cheese and 560 milk samples were collected during the year 2020. All samples were subjected to PCR based on transposable gene IS1111. The results showed that 12.50% (95.00% confidence interval (CI): 9.00 - 16.10%) of Kope cheese and 13.00% (95.00% CI: 10.00 - 17.30%) of milk samples were positive for Cb. There was a significant difference in cheese and milk contaminations with Cb among the defined age groups as well as regional and seasonal variations. It was concluded that Kope cheese and cattle milk are important sources of Cb and should be considered as important risk factors in the epidemiology of Q fever disease in public health. (c) 2023 Urmia University. All rights reserved.
引用
收藏
页码:289 / 293
页数:5
相关论文
共 29 条
[1]   Seroprevalence of Q fever in sheep and goat flocks with a history of abortion in Iran between 2011 and 2012 [J].
Asadi, Javad ;
Kafi, Mojtaba ;
Khalili, Mohammad .
VETERINARIA ITALIANA, 2013, 49 (02) :163-168
[2]   Viable Coxiella burnetii in hard cheeses made with unpasteurized milk [J].
Barandika, Jesus F. ;
Alvarez-Alonso, Raquel ;
Jado, Isabel ;
Hurtado, Ana ;
Garcia-Perez, Ana L. .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2019, 303 :42-45
[3]   The detection of Coxiella burnetii from ovine genital swabs, milk and fecal samples by the use of a single touchdown polymerase chain reaction [J].
Berri, M ;
Laroucau, K ;
Rodolakis, A .
VETERINARY MICROBIOLOGY, 2000, 72 (3-4) :285-293
[4]  
Berri Mustapha, 2003, Methods Mol Biol, V216, P153
[5]  
Boldis V, 2013, Mol. Typ., P457, DOI 10.1007/978-1-62703-185-126
[6]   Evaluation of DNA extraction methods for use in combination with SYBR Green I real-time PCR to detect Salmonella enterica serotype enteritidis in poultry [J].
De Medici, D ;
Croci, L ;
Delibato, E ;
Di Pasquale, S ;
Filetici, E ;
Toti, L .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (06) :3456-3461
[7]  
Delsing CE, 2010, NETH J MED, V68, P382
[8]   From Q Fever to Coxiella burnetii Infection: a Paradigm Change [J].
Eldin, Carole ;
Melenotte, Clea ;
Mediannikov, Oleg ;
Ghigo, Eric ;
Million, Matthieu ;
Edouard, Sophie ;
Mege, Jean-Louis ;
Maurin, Max ;
Raoult, Didier .
CLINICAL MICROBIOLOGY REVIEWS, 2017, 30 (01) :115-190
[9]   Coxiella burnetii DNA, But Not Viable Bacteria, in Dairy Products in France [J].
Eldin, Carole ;
Angelakis, Emmanouil ;
Renvoise, Aurelie ;
Raoult, Didier .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2013, 88 (04) :765-769
[10]   High prevalence and risk factors of Coxiella burnetii in milk of dairy animals with a history of abortion in Iran [J].
Esmaeili, Saber ;
Mobarez, Ashraf Mohabati ;
Khalili, Mohammad ;
Mostafavi, Ehsan .
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 2019, 63 :127-130