Isolation, Identification, and Determination of the Virulence of the Causal Agents of Corm Rot of Saffron (Crocus sativus L.) in Valle de Uco, Argentina

被引:2
|
作者
Caligiore-Gei, Pablo F. [1 ]
Moratalla-Lopez, Natalia [2 ]
Poggi, Luciana M. [1 ]
Alonso, Gonzalo L. [2 ]
机构
[1] Inst Nacl Tecnol Agr, Estn Expt Agr Consulta, Ex Ruta 40 km 96 5, RA-5567 La Consulta, Mendoza, Argentina
[2] Univ Castilla La Mancha, Catedra Quim Agr, ETS Ingn Agron & Montes & Biotecnol Albacete, Campus Univ, Albacete 02071, Spain
来源
PLANTS-BASEL | 2023年 / 12卷 / 14期
关键词
Fusarium oxysporum Schltdl; saffron; fungi; spice; phytosanitary status;
D O I
10.3390/plants12142717
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Saffron (Crocus sativus L.) presents an attractive opportunity for diversifying production and adding value, particularly for small-scale growers and family-based agriculture. However, the agamic propagation of the crop through corms raises concerns regarding disease dispersion. During the summers of 2013 and 2015, symptoms of corm rot were observed in saffron crops in La Consulta, Valle de Uco, Argentina. These symptoms manifested in the form of wilting plants and red-coloured areas on the surface of the corms, in some cases affecting deeper regions. This study aimed to isolate and identify the causal agent responsible for saffron corm rot while also comparing the virulence of four strains isolated on saffron plants. Consistent isolation of Fusarium spp. colonies from affected corms confirmed its association with the disease. The obtained isolates were inoculated into healthy corms, and the reproduction of symptoms was confirmed, as well as subsequent pathogen re-isolation. Morphological and molecular characterisation of the strains was performed using rDNA gene sequencing. Furthermore, disease progression was assessed with fitting epidemiological models to empirical data, which served as estimators of fungal strain aggressiveness. The results conclusively identified Fusarium oxysporum Schltdl. as the causal agent of corm rot, and variations in virulence were observed among the strains on the host plant. After basic molecular and pathological studies, it is postulated that the fungal strains possibly belong to the forma specialis gladioli, but further studies are necessary to confirm that. The present study provides findings that highlight the importance of early detection and the preservation of pathogen-free fields to sustain saffron cultivation. These findings may constitute the initial step for future projects aimed at understanding the epidemiology of the disease better, determining the species/races of the pathogen, and developing effective management strategies.
引用
收藏
页数:10
相关论文
共 45 条
  • [21] Antagonism of Bacillus velezensis ZGE166 Against the Pathogenic Fungi Causing Corm Rot Disease in Saffron (Crocus sativus L.)Antagonism of Bacillus velezensis ZGE166 Against the Pathogenic Fungi Causing Corm Rot Disease in Saffron (Crocus sativus L.)Y. Guo et al.
    Yingqiu Guo
    Li Tian
    Xinyu Zhu
    Shu Liu
    Lili Wang
    Wankui Li
    Microbial Ecology, 88 (1)
  • [22] Rapid determination of safranal in the quality control of saffron spice (Crocus sativus L.)
    Maggi, Luana
    Sanchez, Ana M.
    Carmona, Manuel
    Kanakis, Charalabos D.
    Anastasaki, Eirini
    Tarantilis, Petros A.
    Polissiou, Moschos G.
    Alonso, Gonzalo L.
    FOOD CHEMISTRY, 2011, 127 (01) : 369 - 373
  • [23] The possibility of improving saffron (Crocus sativus L.) flower and corm yield through the irrigation and soil texture managements
    Shajari, Mahsa Aghhavani
    Moghaddam, Parviz Rezvani
    Ghorbani, Reza
    Koocheki, Alireza
    SCIENTIA HORTICULTURAE, 2020, 271
  • [24] In vitro shoot formation and enrooted mini-corm production by direct organogenesis in saffron (crocus sativus L.)
    Lagram K.
    El Merzougui S.
    Boudadi I.
    Ben El Caid M.
    El Boullani R.
    El Mousadik A.
    Serghini M.A.
    Vegetos, 2024, 37 (3): : 1045 - 1050
  • [25] The effect of time of corm lifting and duration of incubation at inductive temperature on flowering in the saffron plant (Crocus sativus L.)
    Molina, R
    Valero, M
    Navarro, Y
    García-Luis, A
    Guardiola, JL
    SCIENTIA HORTICULTURAE, 2004, 103 (01) : 79 - 91
  • [26] Assessment of the Effects of Some Bacterial Isolates and Hormones on Corm Formation and Some Plant Properties in Saffron (Crocus sativus L.)
    Parlakova Karagoz, Fazilet
    Dursun, Atilla
    Kotan, Recep
    Ekinci, Melek
    Yildirim, Ertan
    Mohammadi, Parisa
    JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, 2016, 22 (04): : 500 - 511
  • [27] Evaluation of the Effects of Saffron (Crocus sativus L.) Corm Extract on Growing Indices of Cucurbita pepo var. styrica
    Izadpanah, F.
    Feizi, T.
    Ghomi, S.
    Kalantari, S.
    Hassani, M. E.
    III INTERNATIONAL SYMPOSIUM ON SAFFRON: FORTHCOMING CHALLENGES IN CULTIVATION, RESEARCH AND ECONOMICS, 2010, 850 : 275 - 277
  • [28] THE EFFECTS OF DIFFERENT GIBBERELLIC ACID DOSES AND CORM CUTTING METHODS ON SAFFRON (CROCUS SATIVUS L.) YIELD COMPONENTS IN TURKEY
    Asil, Hasan
    Ayanoglu, Filiz
    FRESENIUS ENVIRONMENTAL BULLETIN, 2018, 27 (12A): : 9222 - 9229
  • [29] Determination of Antioxidant Properties of Six By-Products of Crocus sativus L. (Saffron) Plant Products
    Lahmass, Iliass
    Ouahhoud, Sabir
    Elmansuri, Mohammed
    Sabouni, Assia
    Elyoubi, Mohammed
    Benabbas, Redouane
    Choukri, Mohammed
    Saalaoui, Ennouamane
    WASTE AND BIOMASS VALORIZATION, 2018, 9 (08) : 1349 - 1357
  • [30] The possibility for improvement of flowering, corm properties, bioactive compounds, and antioxidant activity in saffron (Crocus sativus L.) by different nutritional regimes
    Ghanbari, Jalal
    Khajoei-Nejad, Gholamreza
    van Ruth, Saskia M.
    Aghighi, Sonia
    INDUSTRIAL CROPS AND PRODUCTS, 2019, 135 : 301 - 310