Label-free optical interferometric microscopy to characterize morphodynamics in living plants

被引:1
|
作者
Ebrahimi, Samira [1 ,2 ]
Moreno-Pescador, Guillermo [1 ,2 ]
Persson, Staffan [1 ,3 ]
Jauffred, Liselotte [2 ]
Bendix, Poul Martin [2 ]
机构
[1] Univ Copenhagen, Copenhagen Plant Sci Ctr, Dept Plant & Environm Sci, Frederiksberg, Denmark
[2] Univ Copenhagen, Niels Bohr Inst, Biocomplex, Copenhagen, Denmark
[3] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab & Dev Sci, State Key Lab Hybrid Rice, Shanghai, Peoples R China
来源
FRONTIERS IN PLANT SCIENCE | 2023年 / 14卷
关键词
digital holographic cell imaging; optical coherence tomography; label-free microscopy; material transport; intereferometric imaging; plant cells and tissues; speckle imaging; plant morphodynamics; DIGITAL HOLOGRAPHIC MICROSCOPY; COHERENCE TOMOGRAPHY; BIOSPECKLE ACTIVITY; ROOT-GROWTH; REVEALS; DIFFRACTION; DYNAMICS; LEAF; TOOL;
D O I
10.3389/fpls.2023.1156478
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
During the last century, fluorescence microscopy has played a pivotal role in a range of scientific discoveries. The success of fluorescence microscopy has prevailed despite several shortcomings like measurement time, photobleaching, temporal resolution, and specific sample preparation. To bypass these obstacles, label-free interferometric methods have been developed. Interferometry exploits the full wavefront information of laser light after interaction with biological material to yield interference patterns that contain information about structure and activity. Here, we review recent studies in interferometric imaging of plant cells and tissues, using techniques such as biospeckle imaging, optical coherence tomography, and digital holography. These methods enable quantification of cell morphology and dynamic intracellular measurements over extended periods of time. Recent investigations have showcased the potential of interferometric techniques for precise identification of seed viability and germination, plant diseases, plant growth and cell texture, intracellular activity and cytoplasmic transport. We envision that further developments of these label-free approaches, will allow for high-resolution, dynamic imaging of plants and their organelles, ranging in scales from sub-cellular to tissue and from milliseconds to hours.
引用
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页数:8
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