Identification and characterization of extracellular GH3 β-glucosidase from the pink snow mold fungus, Microdochium nivale

被引：0

作者：

Ota, Tomoya ^{[1
]}

Saburi, Wataru ^{[1
]}

Jewell, Linda Elizabeth ^{[2
]}

Hsiang, Tom ^{[3
]}

Imai, Ryozo ^{[4
]}

Mori, Haruhide ^{[1
]}

机构：

[1] Hokkaido Univ, Res Fac Agr, Sapporo, Japan

[2] Agr & Agrifood Canada, St John, NF, Canada

[3] Univ Guelph, Sch Environm Sci, Guelph, ON, Canada

[4] Natl Agr & Food Res Org, Inst Agrobiol Sci, Tsukuba, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2023年 / 87卷 / 07期

关键词：

Microdochium nivale; beta-glucosidase; glycoside hydrolase family 3; substrate specificity; transglucosylation; SUBSTRATE-SPECIFICITY; CRYSTAL-STRUCTURES; GLYCOSIDE; INDUCTION; CELLULASE; ALIGNMENT; BLOOD;

D O I：

10.1093/bbb/zbad044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glycoside hydrolase family 3 (GH3) beta-glucosidase exists in many filamentous fungi. In phytopathogenic fungi, it is involved in fungal growth and pathogenicity. Microdochium nivale is a severe phytopathogenic fungus of grasses and cereals and is the causal agent of pink snow mold, but its beta-glucosidase has not been identified. In this study, a GH3 beta-glucosidase of M. nivale (MnBG3A) was identified and characterized. Among various p-nitrophenyl beta-glycosides, MnBG3A showed activity on d-glucoside (pNP-Glc) and slight activity on d-xyloside. In the pNP-Glc hydrolysis, substrate inhibition occurred (K-is = 1.6 m m), and d-glucose caused competitive inhibition (K-i = 0.5 m m). MnBG3A acted on beta-glucobioses with beta 1-3, -6, -4, and -2 linkages, in descending order of k(cat)/K-m. In contrast, the regioselectivity for newly formed products was limited to beta 1-6 linkage. MnBG3A has similar features to those of beta-glucosidases from Aspergillus spp., but higher sensitivity to inhibitory effects.

引用

页码：707 / 716

页数：10

共 36 条

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