Differential Protein Expression of Taenia crassiceps ORF Strain in the Murine Cysticercosis Model Using Resistant (C57BL/6) Mice

被引:1
作者
Jimenez, Lucia [1 ]
Diaz-Zaragoza, Mariana [1 ,2 ]
Hernandez, Magdalena [3 ]
Navarro, Luz [4 ]
Hernandez-Avila, Ricardo [5 ]
Encarnacion-Guevara, Sergio [3 ]
Ostoa-Saloma, Pedro [5 ]
Landa, Abraham [1 ]
机构
[1] Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, AP 70228,Ciudad Univ, Mexico City 04510, Mexico
[2] Univ Guadalajara, Ctr Univ Valles, Dept Ciencias Salud, Carretera Guadalajara Ameca Km 45-5, Guadalajara 46600, Mexico
[3] Univ Nacl Autonoma Mexico, Ctr Ciencias Genomicas, Ave Univ 565, Cuernavaca 62210, Mexico
[4] Univ Nacl Autonoma Mexico, Fac Med, Dept Fisiol, AP 70228,Ciudad Univ, Mexico City 04510, Mexico
[5] Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, AP 70228,Ciudad Univ, Mexico City 04510, Mexico
关键词
Taenia crassiceps; cysticerci; Th1; response; C57BL; 6; mouse; proteomics; IMMUNE-RESPONSE; IN-VITRO; DISULFIDE-ISOMERASE; SOLIUM; ENOLASE; PARAMYOSIN; PRODUCTS; VACCINE; FAMILY; HOST;
D O I
10.3390/pathogens12050678
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A cysticercosis model of Taenia crassiceps ORF strain in susceptible BALB/c mice revealed a Th2 response after 4 weeks, allowing for the growth of the parasite, whereas resistant C57BL/6 mice developed a sustained Th1 response, limiting parasitic growth. However, little is known about how cysticerci respond to an immunological environment in resistant mice. Here, we show that the Th1 response, during infection in resistant C57BL/6 mice, lasted up to 8 weeks and kept parasitemia low. Proteomics analysis of parasites during this Th1 environment showed an average of 128 expressed proteins; we chose 15 proteins whose differential expression varied between 70 and 100%. A total of 11 proteins were identified that formed a group whose expression increased at 4 weeks and decreased at 8 weeks, and another group with proteins whose expression was high at 2 weeks and decreased at 8 weeks. These identified proteins participate in tissue repair, immunoregulation and parasite establishment. This suggests that T. crassiceps cysticerci in mice resistant under the Th1 environment express proteins that control damage and help to establish a parasite in the host. These proteins could be targets for drugs or vaccine development.
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页数:13
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