Discovery and Characterization of MaK: A Novel Knottin Antimicrobial Peptide from Monochamus alternatus

被引:3
|
作者
Han, Xiaohong [1 ,2 ]
Zhou, Tong [3 ]
Hu, Xinran [1 ,2 ]
Zhu, Yukun [1 ,2 ]
Shi, Zengzeng [1 ,2 ]
Chen, Shi [1 ,2 ]
Liu, Yunfei [1 ,2 ]
Weng, Xiaoqian [1 ,2 ]
Zhang, Feiping [1 ,2 ]
Wu, Songqing [1 ,4 ]
机构
[1] Fujian Agr & Forestry Univ, Coll Forestry, Fuzhou 350002, Peoples R China
[2] Fujian Agr & Forestry Univ, Fujian Prov Univ, Key Lab Integrated Pest Management Ecol Forests, Fuzhou 350002, Peoples R China
[3] Zhejiang Univ, Inst Insect Sci, Coll Agr & Biotechnol, Hangzhou 310058, Peoples R China
[4] Fujian Agr & Forestry, Fujian Coll & Univ Engn Res Inst Conservat & Utili, Fuzhou 350002, Peoples R China
基金
中国国家自然科学基金;
关键词
Monochamus alternatus; Bursaphelenchus xylophilus; knottin-type peptide; MaK; antimicrobial peptide; bioinformatics analysis; bioactivity; PINE WOOD NEMATODE; PSACOTHEASIN; DROSOPHILA; SEQUENCE; DEFENSE; GENE;
D O I
10.3390/ijms242417565
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Knottin-type antimicrobial peptides possess exceptional attributes, such as high efficacy, low vulnerability to drug resistance, minimal toxicity, and precise targeting of drug sites. These peptides play a crucial role in the innate immunity of insects, offering protection against bacteria, fungi, and parasites. Knottins have garnered considerable interest as promising contenders for drug development due to their ability to bridge the gap between small molecules and protein-based biopharmaceuticals, effectively addressing the therapeutic limitations of both modalities. This work presents the isolation and identification of a novel antimicrobial peptide derived from Monochamus alternatus. The cDNA encodes a 56-amino acid knottin propeptide, while the mature peptide comprises only 34 amino acids. We have labeled this knottin peptide as MaK. Using chemically synthesized MaK, we evaluated its hemolytic activity, thermal stability, antibacterial properties, and efficacy against nematodes. The results of this study indicate that MaK is an exceptionally effective knottin-type peptide. It demonstrates low toxicity, superior stability, potent antibacterial activity, and the ability to suppress pine wood nematodes. Consequently, these findings suggest that MaK has potential use in developing innovative therapeutic agents to prevent and manage pine wilt disease.
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收藏
页数:13
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