共 63 条
Structural Dynamics of Protein Interactions Using Site-Directed Spin Labeling of Cysteines to Measure Distances and Rotational Dynamics with EPR Spectroscopy
被引:5
作者:

Roopnarine, Osha
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

Thomas, David D.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA
机构:
[1] Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA
关键词:
NITROXIDE SIDE-CHAINS;
CONFORMATIONAL-CHANGES;
ANCHORED PROTEINS;
ALPHA-SYNUCLEIN;
GLYCOSPHINGOLIPIDS;
GANGLIOSIDES;
RESONANCE;
PEPTIDE;
IDENTIFICATION;
ACCESSIBILITY;
D O I:
10.1007/s00723-023-01623-x
中图分类号:
O64 [物理化学(理论化学)、化学物理学];
O56 [分子物理学、原子物理学];
学科分类号:
070203 ;
070304 ;
081704 ;
1406 ;
摘要:
Here we review applications of site-directed spin labeling (SDSL) with engineered cysteines in proteins, to study the structural dynamics of muscle and non-muscle proteins, using and developing the electron paramagnetic resonance (EPR) spectroscopic techniques of dipolar EPR, double electron electron resonance (DEER), saturation transfer EPR (STEPR), and orientation measured by EPR. The SDSL technology pioneered by Wayne Hubbell and collaborators has greatly expanded the use of EPR, including the measurement of distances between spin labels covalently attached to proteins and peptides. The Thomas lab and collaborators have applied these techniques to elucidate dynamic interactions in the myosin-actin complex, myosin-binding protein C, calmodulin, ryanodine receptor, phospholamban, utrophin, dystrophin, beta-III-spectrin, and Aurora kinase. The ability to design and engineer cysteines in proteins for site-directed covalent labeling has enabled the use of these powerful EPR techniques to measure distances, while showing that they are complementary with optical spectroscopy measurements.
引用
收藏
页码:79 / 100
页数:22
相关论文
共 63 条
[1]
Structural dynamics of the myosin relay helix by time-resolved EPR and FRET
[J].
Agafonov, Roman V.
;
Negrashov, Igor V.
;
Tkachev, Yaroslav V.
;
Blakely, Sarah E.
;
Titus, Margaret A.
;
Thomas, David D.
;
Nesmelov, Yuri E.
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
2009, 106 (51)
:21625-21630

Agafonov, Roman V.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

Negrashov, Igor V.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

Tkachev, Yaroslav V.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA
Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 119991, Russia Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

Blakely, Sarah E.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

Titus, Margaret A.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Sch Med, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

Thomas, David D.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

Nesmelov, Yuri E.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Sch Med, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA
[2]
TRANSMEMBRANE PROTEIN-STRUCTURE - SPIN LABELING OF BACTERIORHODOPSIN MUTANTS
[J].
ALTENBACH, C
;
MARTI, T
;
KHORANA, HG
;
HUBBELL, WL
.
SCIENCE,
1990, 248 (4959)
:1088-1092

ALTENBACH, C
论文数: 0 引用数: 0
h-index: 0
机构: MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA

MARTI, T
论文数: 0 引用数: 0
h-index: 0
机构: MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA

KHORANA, HG
论文数: 0 引用数: 0
h-index: 0
机构: MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA

HUBBELL, WL
论文数: 0 引用数: 0
h-index: 0
机构: MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA
[3]
Structural basis for high-affinity actin binding revealed by a β-III-spectrin SCA5 missense mutation
[J].
Avery, Adam W.
;
Fealey, Michael E.
;
Wang, Fengbin
;
Orlova, Albina
;
Thompson, Andrew R.
;
Thomas, David D.
;
Hays, Thomas S.
;
Egelman, Edward H.
.
NATURE COMMUNICATIONS,
2017, 8

Avery, Adam W.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

Fealey, Michael E.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

Wang, Fengbin
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Virginia, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

Orlova, Albina
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Virginia, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

Thompson, Andrew R.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

Thomas, David D.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

Hays, Thomas S.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

Egelman, Edward H.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Virginia, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA
[4]
A human β-III-spectrin spinocerebellar ataxia type 5 mutation causes high-affinity F-actin binding
[J].
Avery, Adam W.
;
Crain, Jonathan
;
Thomas, David D.
;
Hays, Thomas S.
.
SCIENTIFIC REPORTS,
2016, 6

Avery, Adam W.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

Crain, Jonathan
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

Thomas, David D.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA

Hays, Thomas S.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA
[5]
A large and distinct rotation of the myosin light chain domain occurs upon muscle contraction
[J].
Baker, JE
;
Brust-Mascher, I
;
Ramachandran, S
;
LaConte, LEW
;
Thomas, DD
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1998, 95 (06)
:2944-2949

Baker, JE
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Sch Med, Dept Biochem, Minneapolis, MN 55455 USA Univ Minnesota, Sch Med, Dept Biochem, Minneapolis, MN 55455 USA

Brust-Mascher, I
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Sch Med, Dept Biochem, Minneapolis, MN 55455 USA Univ Minnesota, Sch Med, Dept Biochem, Minneapolis, MN 55455 USA

Ramachandran, S
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Sch Med, Dept Biochem, Minneapolis, MN 55455 USA Univ Minnesota, Sch Med, Dept Biochem, Minneapolis, MN 55455 USA

LaConte, LEW
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Sch Med, Dept Biochem, Minneapolis, MN 55455 USA Univ Minnesota, Sch Med, Dept Biochem, Minneapolis, MN 55455 USA

Thomas, DD
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Sch Med, Dept Biochem, Minneapolis, MN 55455 USA Univ Minnesota, Sch Med, Dept Biochem, Minneapolis, MN 55455 USA
[6]
HIGH-RESOLUTION DETECTION OF MUSCLE CROSSBRIDGE ORIENTATION BY ELECTRON-PARAMAGNETIC RESONANCE
[J].
BARNETT, VA
;
FAJER, P
;
POLNASZEK, CF
;
THOMAS, DD
.
BIOPHYSICAL JOURNAL,
1986, 49 (01)
:144-147

BARNETT, VA
论文数: 0 引用数: 0
h-index: 0

FAJER, P
论文数: 0 引用数: 0
h-index: 0

POLNASZEK, CF
论文数: 0 引用数: 0
h-index: 0

THOMAS, DD
论文数: 0 引用数: 0
h-index: 0
[7]
X-ray structures of the apo and MgATP-bound states of Dictyostelium discoideum myosin motor domain
[J].
Bauer, CB
;
Holden, HM
;
Thoden, JB
;
Smith, R
;
Rayment, I
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2000, 275 (49)
:38494-38499

Bauer, CB
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

Holden, HM
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

Thoden, JB
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

Smith, R
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

Rayment, I
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA
[8]
Macromolecular complexes regulating cardiac ryanodine receptor function
[J].
Bers, DM
.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY,
2004, 37 (02)
:417-429

Bers, DM
论文数: 0 引用数: 0
h-index: 0
机构:
Loyola Univ, Stritch Sch Med, Dept Physiol, Maywood, IL 60153 USA Loyola Univ, Stritch Sch Med, Dept Physiol, Maywood, IL 60153 USA
[9]
A Large-Scale High-Throughput Screen for Modulators of SERCA Activity
[J].
Bidwell, Philip A. A.
;
Yuen, Samantha L. L.
;
Li, Ji
;
Berg, Kaja
;
Rebbeck, Robyn T. T.
;
Aldrich, Courtney C. C.
;
Roopnarine, Osha
;
Cornea, Razvan L. L.
;
Thomas, David D. D.
.
BIOMOLECULES,
2022, 12 (12)

Bidwell, Philip A. A.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Med, Div Cardiovasc, Minneapolis, MN 55455 USA Univ Minnesota, Dept Med, Div Cardiovasc, Minneapolis, MN 55455 USA

Yuen, Samantha L. L.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Med, Div Cardiovasc, Minneapolis, MN 55455 USA

Li, Ji
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Med, Div Cardiovasc, Minneapolis, MN 55455 USA

Berg, Kaja
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Med Chem, Minneapolis, MN 55455 USA Univ Minnesota, Dept Med, Div Cardiovasc, Minneapolis, MN 55455 USA

Rebbeck, Robyn T. T.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Med, Div Cardiovasc, Minneapolis, MN 55455 USA

Aldrich, Courtney C. C.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Med Chem, Minneapolis, MN 55455 USA Univ Minnesota, Dept Med, Div Cardiovasc, Minneapolis, MN 55455 USA

Roopnarine, Osha
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Med, Div Cardiovasc, Minneapolis, MN 55455 USA

Cornea, Razvan L. L.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Med, Div Cardiovasc, Minneapolis, MN 55455 USA

Thomas, David D. D.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Med, Div Cardiovasc, Minneapolis, MN 55455 USA
[10]
High-resolution helix orientation in actin-bound myosin determined with a bifunctional spin label
[J].
Binder, Benjamin P.
;
Cornea, Sinziana
;
Thompson, Andrew R.
;
Moen, Rebecca J.
;
Thomas, David D.
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
2015, 112 (26)
:7972-7977

Binder, Benjamin P.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

Cornea, Sinziana
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

Thompson, Andrew R.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

Moen, Rebecca J.
论文数: 0 引用数: 0
h-index: 0
机构:
Minnesota State Univ, Dept Chem & Geol, Mankato, MN 56001 USA Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

Thomas, David D.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA