Antitumoral Activity of Cecropia Pachystachya Leaves Extract in Vitro and in Vivo Model of Rat Glioma: Brain and Blood Effects

被引：2

作者：

Bona, Natalia Pontes ^{[1
]}

Pedra, Nathalia Stark ^{[2
]}

Spohr, Luiza ^{[2
]}

dos Santos, Francieli da Silva ^{[1
]}

Saraiva, Juliane Torchelsen ^{[1
]}

Carvalho, Fabiano Barbosa ^{[3
]}

Fernandes, Marilda da Cruz ^{[3
]}

Fernandes, Ana Sofia ^{[4
]}

Saraiva, Nuno ^{[4
]}

Martins, Marta Filipa ^{[4
,5
]}

Tavares, Rejane Giacomelli ^{[4
,6
]}

Spanevello, Roselia Maria ^{[2
]}

Aguiar, Mayara Sandrielly Soares de ^{[2
]}

Stefanello, Francieli Moro ^{[1
]}

机构：

[1] Univ Fed Pelotas, Ctr Ciencias Quim Farmaceut & Alimentos, Programa Posgrad Bioquim & Bioprospeccao, Lab Biomarcadores, Campus Univ S-N, BR-96010900 Pelotas, RS, Brazil

[2] Univ Fed Pelotas, Ctr Ciencias Quim Farmaceut & Alimentos, Programa Posgrad Bioquim & Bioprospeccao, Lab Neuroquim Inflamacao & Canc, Campus Univ S-N, Pelotas, RS, Brazil

[3] Univ Fed Ciencias Saude Porto Alegre, Lab Pesquisa Patol, Porto Alegre, RS, Brazil

[4] Univ Lusofonas Res Ctr Biosci & Hlth Technol, CBIOS, Campo Grande 376, P-1749024 Lisbon, Portugal

[5] Univ Alcala, Dept Biomed Sci, Ctra Madrid Barcelona Km 33-600, Alcala De Henares 28871, Spain

[6] Univ Fed Pelotas, Programa Posgrad Multicentr Ciencias Fisiol, Inst Biol, Pelotas, Brazil

来源：

MOLECULAR NEUROBIOLOGY | 2024年 / 61卷 / 10期

关键词：

Glioblastoma; Natural product; Cecropia pachystachya; Toxicity; Redox status; Purinergic system; COLORIMETRIC ASSAY; MONONUCLEAR-CELLS; EXTRACELLULAR ATP; OXIDATIVE STRESS; DIPHOSPHOHYDROLASE; ECTONUCLEOTIDASE; GLIOBLASTOMA; EC-3.6.1.5; SURVIVAL; BEHAVIOR;

D O I：

10.1007/s12035-024-04086-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The aim of this study was to investigate the antiglioma effect of Cecropia pachystachya Trecul (CEC) leaves extract against C6 and U87 glioblastoma (GB) cells and in a rat preclinical GB model. The CEC extract reduced in vitro cell viability and biomass. In vivo, the extract decreased the tumor volume approximately 62%, without inducing systemic toxicity. The deficit in locomotion and memory and an anxiolytic-like behaviors induced in the GB model were minimized by CEC. The extract decreased the levels of reactive oxygen species, nitrites and thiobarbituric acid reactive substances and increased the activity of antioxidant enzymes in platelets, sera and brains of GB animals. The activity of NTPDases, 5'-nucleotidase and adenosine deaminase (ADA) was evaluated in lymphocytes, platelets and serum. In platelets, ATP and AMP hydrolysis was reduced and hydrolysis of ADP and the activity of ADA were increased in the control, while in CEC-treated animals no alteration in the hydrolysis of ADP was detected. In serum, the reduction in ATP hydrolysis was reversed by CEC. In lymphocytes, the increase in the hydrolysis of ATP, ADP and in the activity of ADA observed in GB model was altered by CEC administration. The observed increase in IL-6 and decrease in IL-10 levels in the serum of GB animals was reversed by CEC. These results demonstrate that CEC extract is a potential complementary treatment to GB, decreasing the tumor size, while modulating aspects of redox and purinergic systems.

引用

页码：8234 / 8252

页数：19

共 72 条

[1] Therapeutic potential of natural products in glioblastoma treatment: targeting key glioblastoma signaling pathways and epigenetic alterations [J].