Characterization and functional analyses of wheat TaPR1 genes in response to stripe rust fungal infection

被引：19

作者：

Liu, Rong ^{[1
]}

Lu, Jing ^{[2
]}

Xing, Jiayi ^{[2
]}

Xue, Lv ^{[2
]}

Wu, Yu ^{[2
,3
]}

Zhang, Lei ^{[2
,3
]}

机构：

[1] Yibin Univ, Fac Agr Forestry & Food Engn, Yibin 644000, Peoples R China

[2] Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China

[3] Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China

来源：

SCIENTIFIC REPORTS | 2023年 / 13卷 / 01期

关键词：

F-SP TRITICI; WRKY TRANSCRIPTION FACTOR; SALICYLIC-ACID; DEFENSE; PROTEIN; EXPRESSION; RESISTANCE; INDUCTION; TOBACCO; PR-1;

D O I：

10.1038/s41598-023-30456-8

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The pathogenesis-related protein-1 (PR1) gene is important for plants to respond to various biotic and abiotic stresses. Unlike those in model plants, PR1 genes in wheat have not been systematically studied. Herein, we identified 86 potential TaPR1 wheat genes using bioinformatics tools and RNA sequencing. Kyoto Encyclopedia of Genes and Genomes analysis indicated that the TaPR1 genes were involved in the salicylic acid signalling pathway, MAPK signalling pathway, and phenylalanine metabolism in response to Pst-CYR34 infection. Ten of the TaPR1 genes were structurally characterized and validated by RT-PCR. One particular gene, TaPR1-7, was found to be associated with resistance to Puccinia striiformis f. sp. tritici (Pst) in a biparental wheat population. Virus-induced gene silencing showed that TaPR1-7 is important for Pst resistance in wheat. This study provides the first comprehensive study on wheat PR1 genes, improving our overall understanding of these genes in plant defenses, particularly against stripe rust.

引用

页数：13

共 52 条

[1] A novel rice (Oryza sativa L.) acidic PR1 gene highly responsive to cut, phytohormones, and protein phosphatase inhibitors [J].

Agrawal, GK ;

Jwa, NS ;

Rakwal, R .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 274 (01) :157-165

[2] Pathogenesis related protein-1 (PR-1) genes in tomato (Solanum lycopersicum L.): Bioinformatics analyses and expression profiles in response to drought stress [J].

Akbudak, M. Aydin ;

Yildiz, Sukran ;

Filiz, Ertugrul .

GENOMICS, 2020, 112 (06) :4089-4099

[3] INCREASED TOLERANCE TO 2 OOMYCETE PATHOGENS IN TRANSGENIC TOBACCO EXPRESSING PATHOGENESIS-RELATED PROTEIN-1A [J].

ALEXANDER, D ;

GOODMAN, RM ;

GUTRELLA, M ;

GLASCOCK, C ;

WEYMANN, K ;

FRIEDRICH, L ;

MADDOX, D ;

AHLGOY, P ;

LUNTZ, T ;

WARD, E ;

RYALS, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (15) :7327-7331

[4] HTSeq-a Python']Python framework to work with high-throughput sequencing data [J].

Anders, Simon ;

Pyl, Paul Theodor ;

Huber, Wolfgang .

BIOINFORMATICS, 2015, 31 (02) :166-169

[5] COMPARISON OF 3 PATHOGENESIS-RELATED PROTEINS FROM PLANTS OF 2 CULTIVARS OF TOBACCO INFECTED WITH TMV [J].

ANTONIW, JF ;

RITTER, CE ;

PIERPOINT, WS ;

VANLOON, LC .

JOURNAL OF GENERAL VIROLOGY, 1980, 47 (MAR) :79-87

[6] Host-pathogen interactions Cell death and infection: A double-edged sword for host and pathogen survival [J].

Ashida, Hiroshi ;

Mimuro, Hitomi ;

Ogawa, Michinaga ;

Kobayashi, Taira ;

Sanada, Takahito ;

Kim, Minsoo ;

Sasakawa, Chihiro .

JOURNAL OF CELL BIOLOGY, 2011, 195 (06) :931-942

[7] The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana [J].

Cannon S.B. ;

Mitra A. ;

Baumgarten A. ;

Young N.D. ;

May G. .

BMC Plant Biology, 4 (1)

[8] TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data [J].

Chen, Chengjie ;

Chen, Hao ;

Zhang, Yi ;

Thomas, Hannah R. ;

Frank, Margaret H. ;

He, Yehua ;

Xia, Rui .

MOLECULAR PLANT, 2020, 13 (08) :1194-1202

[9] Race Dynamics, Diversity, and Virulence Evolution in Puccinia striiformis f. sp tritici, the Causal Agent of Wheat Stripe Rust in China from 2003 to 2007 [J].

Chen, W. Q. ;

Wu, L. R. ;

Liu, T. G. ;

Xu, S. C. ;

Jin, S. L. ;

Peng, Y. L. ;

Wang, B. T. .

PLANT DISEASE, 2009, 93 (11) :1093-1101

[10]

Chen XM, 2005, CAN J PLANT PATHOL, V27, P314

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