Effect of Sr, Mg and Fe substitution on the physico-chemical and biological properties of Si Ca P multilayer scaffolds

In this work, a new combination of ceramic materials is proposed for bone tissue engineering applications. Multilayer scaffolds consisting of a core composed mainly of calcium pyrophosphate and external coatings of silica and calcium doped with Fe3+, Sr2+ and Mg2+ were prepared. To study the influence of the arrangement of dopant ions in the external coatings, two different scaffolds were developed: scaffolds 3J consisting of a single external coating with 9 mol% of Fe3+, Sr2+ and Mg2+ ions; and scaffolds 3S comprising three external coatings, each containing 3 mol% of Fe3+, Sr2+ and Mg2+ ions. Scaffolds were physicochemically characterized and evaluated for in vitro bioactivity and cellular response in the presence of MG-63 cells. The results showed that the core scaffold displayed no in vitro bioactivity or good cellular response, but served as a support for the external coatings given its mechanical resistance. The cell viability of scaffolds 3J and 3S increased more than 100% in relation to the core, and also improved cell proliferation and adhesion resulting in a dense layer of cells that covered the scaffolds' entire surface. The arrangement of ions in the external coatings did not influence the cellular response, but determined the bioactivity rate. (c) 2021 SECV. Published by Elsevier Espa n similar to a, S.L.U. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).