Tuina Intervention in Rabbit Model of Knee Osteoarthritis

被引：2

作者：

Zhang, Shidong ^{[1
]}

Zhang, Xuan ^{[2
]}

Sun, Gangyi ^{[3
]}

Wang, Kai ^{[1
]}

Qiao, Yingjie ^{[1
]}

He, Yanling ^{[3
]}

Li, Muzhen ^{[1
]}

Li, Huadong ^{[3
]}

Zheng, Lijun ^{[3
]}

机构：

[1] Shandong Univ Tradit Chinese Med, Jinan, Peoples R China

[2] Liaoning Univ Tradit Chinese Med, Liaoning, Peoples R China

[3] Shandong Univ Tradit Chinese Med, Affiliated Hosp, Jinan, Peoples R China

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2023年 / 198期

关键词：

TRADITIONAL CHINESE MEDICINE; RESISTANCE EXERCISE; ADDUCTION MOMENT; GAIT; CARTILAGE; PROGRESSION; INJECTION; MECHANISM; TURNOVER; EFFICACY;

D O I：

10.3791/65763

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Knee osteoarthritis (KOA) is mainly characterized by degenerative changes in the knee joint's cartilage and surrounding soft tissues. The efficacy of Tuina in treating KOA has been confirmed, but the underlying mechanism needs to be investigated. This study aims to establish a scientifically feasible KOA rabbit model treated with Tuina to reveal the underlying mechanisms. For this, 18, 6-month-old normal-grade male New Zealand rabbits were randomly divided into sham, model, and Tuina groups, with 6 rabbits in each group. The KOA model was established by injecting 4% papain solution into the knee joint cavity. The Tuina group was intervened with Tuina combined with the knee joint rotary correction method for 4 weeks. Only the standard grasping and fixation were performed in sham and model groups. At the end of the 1-week intervention, the knee joint range of motion (ROM) was observed, and cartilage hematoxylin-eosin (HE) staining was done. The study shows that Tuina could inhibit chondrocyte apoptosis, repair cartilage tissue, and restore knee joint ROM. In conclusion, this study demonstrates the scientific feasibility of Tuina treatment for KOA model rabbits, highlighting its potential application in the study of KOA and similar knee joint-related conditions.

引用

页数：15

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