Molecular characterization and prevalence of Cryptosporidium spp. in sheep and goats in western Inner Mongolia, China

被引：10

作者：

Lang, Jiashu ^{[1
,2
,3
]}

Han, Han ^{[4
]}

Dong, Heping ^{[5
]}

Qin, Ziyang ^{[1
,2
,3
]}

Fu, Yin ^{[1
,2
,3
]}

Qin, Huikai ^{[1
,2
,3
]}

Zhang, Junchen ^{[1
,2
,3
]}

Zhao, Jinfeng ^{[1
,2
,3
]}

Li, Xiaoying ^{[1
,2
,3
]}

Zhao, Guanghui ^{[5
]}

Li, Junqiang ^{[1
,2
,3
]}

Zhang, Longxian ^{[1
,2
,3
]}

机构：

[1] Henan Agr Univ, No Longzihu Univ Area 15, Coll Vet Med, Zhengzhou New Dist, Zhengzhou 450046, Peoples R China

[2] Int Joint Res Lab Zoonot Dis Henan, Zhengzhou 450046, Peoples R China

[3] Minist Agr & Rural Affairs, Key Lab Qual & Safety Control Poultry Prod Zhengzh, Zhengzhou, Peoples R China

[4] Norman Bethune Hlth Sci Ctr Jilin Univ, Changchun 130021, Peoples R China

[5] Northwest A&F Univ, Coll Vet Med, Yangling 712100, Peoples R China

来源：

PARASITOLOGY RESEARCH | 2023年 / 122卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Cryptosporidium; Sheep; Goats; SSU rRNA; gp60; Zoonotic; INFECTION; UBIQUITUM; LAMBS; SPP;

D O I：

10.1007/s00436-022-07756-5

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Cryptosporidium spp. are zoonotic intestinal parasites that infect fish, birds, reptiles and mammals. Cryptosporidium spp. are common cause of diarrhea. In this study, a total of 1032 fecal samples were collected from the rectums of sheep and goats. The samples were analyzed using nested polymerase chain reaction (nested PCR) based on the small subunit ribosomal RNA (SSU rRNA) gene of Cryptosporidium spp. The average infection rate of Cryptosporidium spp. was 2.23% (n = 23), and three Cryptosporidium species were identified, namely Cryptosporidium ubiquitum (8/23), Cryptosporidium andersoni (5/23) and Cryptosporidium xiaoi (10/23). Subtyping of C. ubiquitum and C. xiaoi was carried out by DNA sequence analysis of the 60-kDa glycoprotein (gp60) gene. Eight C. ubiquitum isolates were identified as zoonotic subtype XIIa. Nine C. xiaoi isolates were identified as subtypes XXIIIc (n = 1), XXIIIf (n = 3) and XXIIIg (n = 5). Subtype XXIIIg was first found in Chinese sheep. C. ubiquitum subtype XIIa was found in both sheep and goats, suggesting that sheep and goats are important sources of C. ubiquitum infections.

引用

页码：537 / 545

页数：9

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