Localization and expression of phospholipase A2 and polyunsaturated fatty acid profile in the testis tissues of Hu sheep

被引：4
作者：
Liang, Weili [1 ,2 ,3 ]
Zhao, Yanhong [4 ]
Quan, Guodong [4 ]
Yao, Rongyu [1 ,2 ,3 ]
Chen, Hua [3 ,5 ]
Weng, Xiuxiu [1 ,2 ,3 ]
Li, Wanhong [1 ,2 ,3 ]
Yue, Xiangpeng [1 ,2 ,3 ]
Li, Fadi [1 ,2 ,3 ,6 ]
机构：
[1] Lanzhou Univ, State Key Lab Herbage Improvement & Grassland Agro, Lanzhou 730020, Peoples R China
[2] Lanzhou Univ, Engn Res Ctr Grassland Ind, Key Lab Grassland Livestock Ind Innovat Minist Agr, Minist Educ, Lanzhou 730020, Peoples R China
[3] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Peoples R China
[4] Tianzhu Cty Anim Breeding Res Inst, Tianzhu 733200, Peoples R China
[5] Gansu Lantiantonghe Agr Co Ltd, Tianzhu 733200, Peoples R China
[6] Gansu Runmu Biol Engn Co Ltd, Yongchang 737200, Peoples R China
来源：
ANIMAL REPRODUCTION SCIENCE | 2024年 / 260卷
关键词：
Hu sheep; Phospholipase A 2; Fatty acid; Testis; ARACHIDONIC-ACID; STIMULATED STEROIDOGENESIS; DIETARY SUPPLEMENTATION; A(2); INVOLVEMENT; LACTATE; OIL;
D O I：
10.1016/j.anireprosci.2023.107381
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The fatty acid content and the localization and expression of phospholipase A(2) (PLA(2)) in the testis of Hu sheep were investigated. A total of 18 six-month-old Hu sheep were divided into small group (S, with left testis weight < 50 g), medium group (M, with left testis weight among 90-110 g), and large group (L, with left testis weight >160 g), which had six individuals each. The expression of PLA(2) in testicular tissues of different sizes was analyzed by immunohistochemistry, RT-qPCR, and Western blot. The fatty acid profile was detected by gas chromatography. Immunohistochemical labeling determined that PLA(2) protein was expressed in the Leydig and Sertoli cells of testis, and the immunohistochemical average optional density in the S group was significantly greater than the L group (P < 0.05). RT-qPCR and Western blot analysis showed that PLA(2) in the S group was greater than that in the L group (P < 0.05). Docosahexaenoic acid, omega-3 polyunsaturated fatty acid (PUFA), and total PUFA content in the testis of the L group were significantly less than those of the S and M groups (P < 0.01). This study showed that PLA(2) content in the S group was greater than that in the L group.
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页数：9
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