Advances in multimodal mass spectrometry for single-cell analysis and imaging enhancement

Multimodal mass spectrometry (MMS) incorporates an imaging modality with probe-based mass spectrometry (MS) to enable precise, targeted data acquisition and provide additional biological and chemical data not available by MS alone. Two categories of MMS are covered; in the first, an imaging modality guides the MS probe to target individual cells and to reduce acquisition time by automatically defining regions of interest. In the second category, imaging and MS data are coupled in the data analysis pipeline to increase the effective spatial resolution using a higher resolution imaging method, correct for tissue deformation, and incorporate fine morphological features in an MS imaging dataset. Recent methodological and computational developments are covered along with their application to single-cell and imaging analyses. Recent developments are highlighted for multimodal mass spectrometry; this includes approaches integrating microscopy and probe-based mass spectrometry for targeted analysis and approaches providing mass spectrometry spatial resolution enhancements for samples ranging from single organelles, individual cells, and tissues.image