ZIKV Strains Elicit Different Inflammatory and Anti-Viral Responses in Microglia Cells

被引:7
作者
de Oliveira, Fernanda Bellaniza Caminha [1 ]
Freire, Vanessa Paola Alves Sampaio de Sa [1 ]
Coelho, Sharton Vinicius Antunes [2 ]
Meuren, Lana Monteiro [2 ]
Palmeira, Julys da Fonseca [1 ]
Cardoso, Ana Luisa [3 ]
Neves, Francisco de Assis Rocha [4 ]
Ribeiro, Bergmann Morais [5 ]
Arganaraz, Gustavo Adolfo [1 ]
Arruda, Luciana Barros de [2 ]
Arganaraz, Enrique Roberto [1 ]
机构
[1] Univ Brasilia, Fac Hlth Sci, Dept Pharm, Lab Mol Neurovirol, BR-70910900 Brasilia, DF, Brazil
[2] Univ Fed Rio de Janeiro, Lab Genet & Imunol Infeccoes Virais, Dept Virol, Inst Microbiol Paulo de Goes, BR-21941902 Rio De Janeiro, RJ, Brazil
[3] Univ Coimbra, Ctr Neurosci & Cell Biol, P-3004517 Coimbra, Portugal
[4] Univ Brasilia, Fac Hlth Sci, Lab Mol Pharmacol, BR-70910900 Brasilia, DF, Brazil
[5] Univ Brasilia, Cell Biol Dept, Lab Bacuolovirus, BR-70910900 Brasilia, DF, Brazil
来源
VIRUSES-BASEL | 2023年 / 15卷 / 06期
关键词
Zika virus; miRNA; PPAR-gamma; microglia; inflammatory response; I INTERFERON-PRODUCTION; MOUSE MODEL; VIRUS; EXPRESSION; ACTIVATION; DENGUE; GAMMA; MACROPHAGES; MECHANISMS; SUPPRESSOR;
D O I
10.3390/v15061250
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In recent years, the Zika Virus (ZIKV) has caused pandemic outbreaks associated with a high rate of congenital ZIKV syndrome (CZS). Although all strains associated with worldwide outbreaks derive from the Asian lineage, the reasons for their enhanced spread and severity are not fully understood. In this study, we conducted a comparative analysis of miRNAs (miRNA-155/146a/124) and their cellular targets (SOCS1/3, SHP1, TRAF6, IRAK1), as well as pro- and anti-inflammatory and anti-viral cytokines (IL-6, TNF-alpha, IFN-gamma, IL-10, and IFN-beta) and peroxisome proliferator-activated receptor gamma (PPAR-gamma) expression in BV2 microglia cells infected with ZIKV strains derived from African and Asian lineages (ZIKV(MR766) and ZIKV(PE243)). BV2 cells were susceptible to both ZIKV strains, and showed discrete levels of viral replication, with delayed release of viral particles without inducing significant cytopathogenic effects. However, the ZIKV(MR766) strain showed higher infectivity and replicative capacity, inducing a higher expression of microglial activation markers than the ZIKV(PE243) strain. Moreover, infection with the ZIKV(MR766) strain promoted both a higher inflammatory response and a lower expression of anti-viral factors compared to the ZIKV(PE243) strain. Remarkably, the ZIKK(PE243) strain induced significantly higher levels of the anti-inflammatory nuclear receptor-PPAR-gamma. These findings improve our understanding of ZIKV-mediated modulation of inflammatory and anti-viral innate immune responses and open a new avenue to explore underlining mechanisms involved in the pathogenesis of ZIKV-associated diseases.
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页数:22
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