Small ruminant lentivirus capsid protein (SRLV-p25) antigenic structural prediction and immunogenicity to recombinant SRLV-rp25-coupled to immunostimulatory complexes based on glycyrrhizinic acid

被引：6
作者：
Azucena Castaneda-Montes, Maria [1 ]
Sandra Cuevas-Romero, Julieta [2 ]
Luis Cerriteno-Sanchez, Jose [2 ]
De Maria Avila-De La Vega, Lucero [1 ]
Bryan Garcia-Cambron, Jose [2 ]
Ramirez-Alvarez, Hugo [1 ]
机构：
[1] Univ Nacl Autonoma Mexico, Fac Higher Educ Cuautitlan Vet Med, Virol Genet & Mol Biol Lab, Campus 4,Km 2-5 Ctra Cuautitlan Teoloyucan, Cuautitlan, Estado De Mexic, Mexico
[2] INIFAP, Lab Virol, Ctr Nacl Invest Disciplinaria Salud Anim & Inocui, KM 15-5 Carretera Mexico Toluca, Ciudad De Mexico, Mexico
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2023年 / 87卷 / 03期
关键词：
liposomes; glycyrrhizinic acid; capsid protein; small ruminant; lentivirus; VIRUS ENVELOPE GENE; HEMAGGLUTININ-NEURAMINIDASE; MUCOSAL IMMUNIZATION; DRUG-DELIVERY; DNA VACCINE; ADJUVANT; MODEL; LIPOSOMES; SHEEP; INDUCTION;
D O I：
10.1093/bbb/zbac206
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Small ruminant lentiviruses (SRLV) infect sheep and goats resulting in significant economic losses. This study evaluated for the first time the predicted conformational structure of the SRLV-capsid-protein 25 (SRLV-p25) and analyzed the antigenicity of recombinant protein (SRLV-rp25) in mice by coupling to an immunostimulatory complexes based on glycyrrhizinic acid liposomes (GAL) and tested plasma from goats and sheep naturally infected. Analysis in silico and conformational structure of SRLV-p25 (genotype B-FESC-752) showed similar characteristics to other lentiviral capsids. The efficient expression of SRLV-rp25 was confirmed by Western blot. The humoral immune responses in mice showed an increased level of antibodies from day 21 to 35 of the SRLV-rp25-GAL and SRLV-rp25-ISCOM (R) groups and the cellular immune response showed no significant difference in IL-10 levels (P >.05), however, a significant difference (P <.001) was observed when comparing SRLV-rp25-GAL with SRLV-rp25 groups. Immunoreactivity toward SRLV-rp25 revealed 61% of positive samples from naturally infected goats and sheep.
引用
收藏
页码：267 / 278
页数：12
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