An instructional workflow for using terrestrial laser scanning (TLS) to quantify vegetation structure for wildlife studies

Vegetation structure is a crucial component of habitat suitability for wildlife, but methods traditionally used to quantify it can be costly, subjective, or difficult to replicate. There have been substantial advancements, and growing interest, in the use of terrestrial laser scanning (TLS) methods to measure fine-scale vegetation attributes in ecological research, but limited opportunities for ecologists and practitioners to learn how to incorporate these into applied research. Here, we provide a starting point for those who want to incorporate TLS methods into ecological research and monitoring, using a case study from a temperate forest in South-Eastern Australia. We scanned 24 sites at different stages of post-fire recovery as part of a larger project measuring the impact of fire on forest dwelling bats, using a FARO Focus 3DS. Using the data from these scans, we present an example workflow with accompanying R code to demonstrate how to process the point clouds and extract a range of vegetation structure metrics relevant to habitat structure for wildlife, including vegetation height, cover, density, and heterogeneity. We discuss how three-dimensional data obtained through TLS can be valuable to wildlife studies and opens up the potential to explore advanced ecological and conservation questions around how vegetation structure influences wildlife behaviours, distribution patterns, and responses to disturbance.