Rational design of antimicrobial peptide conjugated graphene-silver nanoparticle loaded chitosan wound dressing

被引:17
作者
Choudhary, Priyadarshani [1 ,2 ]
Ramalingam, Baskaran [1 ,3 ]
Das, Sujoy K. [2 ,4 ]
机构
[1] CSIR, Cent Leather Res Inst CLRI, Biol Mat Lab, Chennai 600020, India
[2] Acad Sci & Innovat Res AcSIR, Ghaziabad 201002, India
[3] Anna Univ, Dept Civil Engn, Chennai 600020, India
[4] CSIR, Indian Inst Chem Biol IICB, Infect Dis & Immunol Div, Kolkata 700032, India
关键词
Graphene; Chitosan; Hydrogel film; Antimicrobial; Angiogenesis; Wound healing; COMPOSITE FILM; IN-VITRO; HYDROGEL; BACTERIA; ANGIOGENESIS; POLYMER; NANOFIBERS; SCAFFOLD; STRENGTH; METAL;
D O I
10.1016/j.ijbiomac.2023.125347
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Wound dressing with poor antibacterial properties, the tendency to adhere to the wound site, poor mechanical strength, and lack of porosity and flexibility are the major cause of blood loss, delayed wound repair, and sometimes causes death during the trauma or injury. In such cases, hydrogel-based antibacterial wound dressing would be a boon to the existing dressing as the moist environment will maintain the cooling temperate and proper exchange of atmosphere around the wound. In the present study, the multifunctional graphene with silver and & epsilon;-Poly-L-lysine reinforced into the chitosan matrix (CGAPL) was prepared as a nanobiocomposite wound dressing. The contact angle measurement depicted the hydrophilic property of CGAPL nanobiocomposite dressing (water contact angle 42 degrees), while the mechanical property was 78.9 MPa. The antibacterial and cell infiltration study showed the antimicrobial property of CGAPL nanobiocomposite wound dressing. It also demonstrated no cytotoxicity to the L929 fibroblast cells. Chorioallantoic Membrane (CAM) assay showed the pro-angiogenic potential of CGAPL nanobiocomposite wound dressing. In-vitro scratch wound assay confirmed the migration of cells and increased cell adhesion and proliferation within 18 h of culture on the surface of CGAPL nanobiocomposite dressing. Later, the in-vivo study in the Wistar rat model showed that CGAPL nanobiocomposite dressing significantly enhanced the wound healing process as compared to the commercially available wound dressing Tegaderm (p-value <0.01) and Fibroheal@Ag (p-value <0.005) and obtained complete wound closure in 14 days. Histology study further confirmed the complete healing process, re-epithelization, and thick epidermis tissue formation. The proposed CGAPL nanobiocomposite wound dressing thus offers a novel wound dressing material with an efficient and faster wound healing property.
引用
收藏
页数:16
相关论文
共 87 条
[1]   Basic fibroblast growth factor promotes apoptosis and suppresses granulation tissue formation in acute incisional wounds [J].
Akasaka, Y ;
Ono, I ;
Yamashita, T ;
Jimbow, K ;
Ishii, T .
JOURNAL OF PATHOLOGY, 2004, 203 (02) :710-720
[2]  
[Anonymous], 2016, ClinMed Int Library
[3]  
[Anonymous], 2008, ASTM INT, P1
[4]  
[Anonymous], 2003, ASTM INT, V638, P1
[5]   Electrospun chitosan-based nanofibers and their cellular compatibility [J].
Bhattarai, N ;
Edmondson, D ;
Veiseh, O ;
Matsen, FA ;
Zhang, MQ .
BIOMATERIALS, 2005, 26 (31) :6176-6184
[6]   Hydrophilic Graphene Preparation from Gallic Acid Modified Graphene Oxide in Magnesium Self-Propagating High Temperature Synthesis Process [J].
Cao, Lei ;
Li, Zhenhuan ;
Su, Kunmei ;
Cheng, Bowen .
SCIENTIFIC REPORTS, 2016, 6
[7]   The murine excisional wound model: Contraction revisited [J].
Chen, Lin ;
Mirza, Rita ;
Kwon, Young ;
DiPietro, Luisa A. ;
Koh, Timothy J. .
WOUND REPAIR AND REGENERATION, 2015, 23 (06) :874-877
[8]   Fabrication of Nontoxic Reduced Graphene Oxide Protein Nanoframework as Sustained Antimicrobial Coating for Biomedical Application [J].
Choudhary, Priyadarshani ;
Parandhaman, Thanusu ;
Ramalingam, Baskaran ;
Duraipandy, Natarajan ;
Kiran, Manikantan Syamala ;
Das, Sujoy K. .
ACS APPLIED MATERIALS & INTERFACES, 2017, 9 (44) :38255-38269
[9]   Swelling characteristics and biocompatibility of ionic liquid based hydrogels for biomedical applications [J].
Claus, Johanna ;
Brietzke, Andreas ;
Lehnert, Celina ;
Oschatz, Stefan ;
Grabow, Niels ;
Kragl, Udo .
PLOS ONE, 2020, 15 (04)
[10]   Bioinspired self-healing materials: lessons from nature [J].
Cremaldi, Joseph C. ;
Bhushan, Bharat .
BEILSTEIN JOURNAL OF NANOTECHNOLOGY, 2018, 9 :907-935